PF3D7_0809600

Genome location: Pf3D7_08_v3:479,438..497,431(-)

Genome classification: Core

Function and Localization

Product Description: peptidase family C50, putative

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 3.4.22.49 (Separase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005813	centrosome
Component	GO:0005737	cytoplasm
Component	GO:0072686	mitotic spindle
Component	GO:0005634	nucleus
Function	GO:0004197	cysteine-type endopeptidase activity
Process	GO:0051307	meiotic chromosome separation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.13	0.94
Trophozoite	expressed	1.02	0.51
Schizont	expressed	0.47	0.74
Gametocyte	expressed	1.57	0.32

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0809600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.134 | MFS: -2.931 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1427800)

Relative Growth Rate: 0.25 ± 0.24
Confidence: 4.22

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.22.49	separase	actinCdc55astrinpeptidesecurincyclin Bsecuringsercurincyclin B1shugoshinCdk1 kinasesecurin Pds1...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_150828

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	69	199	123	229
disruptive	216	599	319	770
missense	157	472	272	623

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	99	389	170	330
disruptive	393	1307	566	1423
missense	239	933	447	931

PlasmoDB Total SNPs: 614

Non-coding: 62 | Synonymous: 326 | Nonsynonymous: 226 | Stop Codon: 0

Protein Information

Protein Length: 5800 | Molecular Weight (kDa): 698.191

UniProt ID(s): C0H4T3, Q2KNP7, Q2KNQ1, Q2KNT7, Q2KNT9

PDB ID(s): None

Isoelectric Point: 8.26

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR005314	Peptidase C50, separase
PFam	PF03568	N/A
Superfamily	N/A	N/A