About Gene List

PF3D7_0809600

Genome location: Pf3D7_08_v3:479,438..497,431(-)

Genome classification: Core

Function and Localization

Product Description: peptidase family C50, putative

SignalP Peptide: N/A

# Transmembrane Domains: 5

EC Numbers: 3.4.22.49 (Separase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005813 centrosome
Component GO:0005737 cytoplasm
Component GO:0072686 mitotic spindle
Component GO:0005634 nucleus
Function GO:0004197 cysteine-type endopeptidase activity
Process GO:0051307 meiotic chromosome separation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring possibly expressed 0.13 0.94
Trophozoite expressed 1.02 0.51
Schizont expressed 0.47 0.74
Gametocyte expressed 1.57 0.32

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0809600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.134 | MFS: -2.931 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1427800)

  • Relative Growth Rate: 0.25 ± 0.24
  • Confidence: 4.22

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: N/A

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.4.22.49 separase actinCdc55astrinpeptidesecurincyclin Bsecuringsercurincyclin B1shugoshinCdk1 kinasesecurin Pds1...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_150828

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 69 199 123 229
disruptive 216 599 319 770
missense 157 472 272 623

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 99 389 170 330
disruptive 393 1307 566 1423
missense 239 933 447 931

PlasmoDB Total SNPs: 614

Non-coding: 62 | Synonymous: 326 | Nonsynonymous: 226 | Stop Codon: 0

Protein Information

Protein Length: 5800 | Molecular Weight (kDa): 698.191

UniProt ID(s): C0H4T3, Q2KNP7, Q2KNQ1, Q2KNT7, Q2KNT9

PDB ID(s): None

Isoelectric Point: 8.26

Protein Domain Annotations:

Source Family ID Description
InterPro IPR005314 Peptidase C50, separase
PFam PF03568 N/A
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104