Product Description: RuvB-like helicase 1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.12 (DNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0031011 | Ino80 complex |
| Component | GO:0035267 | NuA4 histone acetyltransferase complex |
| Component | GO:0097255 | R2TP complex |
| Component | GO:0000812 | Swr1 complex |
| Function | GO:0043139 | 5'-3' DNA helicase activity |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003678 | DNA helicase activity |
| Process | GO:0000492 | box C/D snoRNP assembly |
| Process | GO:0006338 | chromatin remodeling |
| Process | GO:0016573 | histone acetylation |
| Process | GO:0006357 | regulation of transcription by RNA polymerase II |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.01 | 0.99 |
| Trophozoite | not expressed | 0.03 | 0.98 |
| Schizont | not expressed | 0.06 | 0.95 |
| Gametocyte | expressed | 0.57 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0809700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.999 | MFS: -2.607 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1427700)
Modification: Disrupted | RMgm-5031
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IAT2
"Best" AlphaFill ligand hit: 3AT (cordycepin triphosphate, Local RMSD=0.13) with 6K0R (Global RMSD=3.28)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.12 | DNA helicase | No BRENDA inhibitors |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9Y265 | RuvB-like 1 | OMA, OrthoDB | CHEMBL3263107CHEMBL5178717CHEMBL3263107 |
Protein Length: 520 | Molecular Weight (kDa): 59.595
PDB ID(s): None
Isoelectric Point: 8.05
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR010339 | TIP49, P-loop domain |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR027238 | RuvB-like |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR037938 | RuvB-like helicase 1 |
| InterPro | IPR041048 | RuvB-like, AAA-lid domain |
| PFam | PF06068 | TIP49, P-loop domain |
| PFam | PF17856 | RuvB-like, AAA-lid domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 20 | 8 | 26 |
| disruptive | 5 | 16 | 15 | 38 |
| missense | 5 | 16 | 15 | 30 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 36 | 16 | 33 |
| disruptive | 16 | 46 | 32 | 73 |
| missense | 10 | 39 | 30 | 57 |
PlasmoDB Total SNPs: 96
Non-coding: 73 | Synonymous: 16 | Nonsynonymous: 7 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23219504 | Plasmodium falciparum RuvB1 is an active DNA helicase and translocates in the5'-3' direction. | Ahmad M, Tuteja R | 10.1016/j.gene.2012.11.020 |
| 24161468 | Plasmodium falciparum RuvB2 translocates in 5'-3' direction, relocalizes duringschizont stage and its enzymatic activities are up regulated by RuvB3 of the samecomplex. | Ahmad M, Tuteja R | 10.1016/j.bbapap.2013.10.010 |
| 24934654 | Identification of inhibitors of Plasmodium falciparum RuvB1 helicase usingbiochemical assays | Ahmad M, Tarique M, ..., Tuteja R | 10.1007/s00709-014-0664-6 |