PF3D7_0810400 (AQP2)

Genome location: Pf3D7_08_v3:532,220..534,031(+)

Genome classification: Core

Function and Localization

Product Description: aquaporin, putative

SignalP Peptide: N/A

# Transmembrane Domains: 7

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016021	integral component of membrane
Component	GO:0005886	plasma membrane
Function	GO:0015250	water channel activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.15	0.93
Trophozoite	expressed	0.70	0.61
Schizont	expressed	0.22	0.86
Gametocyte	expressed	0.69	0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0810400

Resistome Missense Mutations: D129Y

Resistome Compounds with Missense Mutations: Suloctidil

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.882 | MFS: 0.263 | #Insertions: 1

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1427100)

Relative Growth Rate: 1.01 ± 0.13
Confidence: 5.42

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1427100)

Modification: Tagged | RMgm-5355

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4T7

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533220

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	13	14	26
disruptive	6	29	25	53
missense	6	28	24	47

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	39	22	30
disruptive	12	79	75	154
missense	8	62	61	112

PlasmoDB Total SNPs: 26

Non-coding: 0 | Synonymous: 18 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 603 | Molecular Weight (kDa): 71.625

UniProt ID(s): C0H4T7

PDB ID(s): None

Isoelectric Point: 9.11

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000425	Major intrinsic protein
InterPro	IPR023271	Aquaporin-like
InterPro	IPR034294	Aquaporin transporter
PFam	PF00230	Major intrinsic protein
Superfamily	SSF81338	Aquaporin-like