Product Description: ATP-dependent RNA helicase DBP1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0043186 | P granule |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003724 | RNA helicase activity |
| Function | GO:0003729 | mRNA binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.63 | 0.70 |
| Trophozoite | expressed | 0.86 | 0.55 |
| Schizont | expressed | 0.15 | 0.91 |
| Gametocyte | expressed | 0.57 | 0.68 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0810600
Resistome Missense Mutations: A414S
Resistome Compounds with Missense Mutations: Artemisinin
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.993 | #Insertions: 5
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1426900)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1426900)
Modification: Disrupted | RMgm-3871
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IAU1
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.02) with 6B4K (Global RMSD=4.24)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| O00571 | ATP-dependent RNA helicase DDX3X | Homo sapiens | OrthoDB | CHEMBL456198CHEMBL2011806CHEMBL2011807CHEMBL2011808... |
Ortholog Group (OrthoMCL): OG6_100461
Most Similar Human Ortholog: A0A2R8YDT5
TM-align score: 0.81 | RMSD: 2.07
Seq Identity: 0.51 | Length: 403 / 941
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000067048 | DEAD-box helicase 3 Y-linked |
| ENSG00000152670 | DEAD-box helicase 4 |
| ENSG00000215301 | DEAD-box helicase 3 X-linked |
Protein Length: 941 | Molecular Weight (kDa): 108.635
UniProt ID(s): A7U5X1, Q19NT7, Q4F6Y9, Q8IAU1
PDB ID(s): None
Isoelectric Point: 9.4
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000629 | ATP-dependent RNA helicase DEAD-box, conserved site |
| InterPro | IPR001650 | Helicase, C-terminal |
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| PFam | PF00271 | Helicase, C-terminal |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 20 | 10 | 34 |
| disruptive | 30 | 63 | 21 | 71 |
| missense | 15 | 36 | 17 | 55 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 61 | 18 | 56 |
| disruptive | 46 | 141 | 93 | 240 |
| missense | 24 | 88 | 74 | 140 |
PlasmoDB Total SNPs: 308
Non-coding: 228 | Synonymous: 61 | Nonsynonymous: 19 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 27381095 | The mRNA-bound proteome of the human malaria parasite Plasmodium falciparum. | Bunnik EM, Batugedara G, ..., Le Roch KG | 10.1186/s13059-016-1014-0 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 36824754 | Comparative transcriptomics reveal differential gene expression in Plasmodiumvivax geographical isolates and implications on erythrocyte invasion mechanisms | Kepple D, Ford CT, ..., Lo E | 10.1101/2023.02.16.528793 |
| 34592986 | The genome of the zoonotic malaria parasite Plasmodium simium reveals adaptationsto host switching | Mourier T, de Alvarenga DAM, ..., Pain A | 10.1186/s12915-021-01139-5 |
| 37667796 | The RNA helicases Dbp2 and Mtr4 regulate the expression of Xrn1-sensitive longnon-coding RNAs in yeast | Wery M, Szachnowski U, ..., Morillon A | 10.3389/frnar.2023.1244554 |
| 32630804 | Hotspots in Plasmodium and RBC Receptor-Ligand Interactions: Key Pieces forInhibiting Malarial Parasite Invasion | Patarroyo MA, Molina-Franky J, ..., Patarroyo ME | 10.3390/ijms21134729 |
| 30872477 | Transcriptome profiling of Plasmodium vivax in Saimiri monkeys identifiespotential ligands for invasion | Gunalan K, Sa JM, ..., Miller LH | 10.1073/pnas.1818485116 |