PF3D7_0811000 (CUL1)

Genome location: Pf3D7_08_v3:553,116..558,148(+)

Genome classification: Core

Function and Localization

Product Description: cullin-1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0019005	SCF ubiquitin ligase complex
Component	GO:0031461	cullin-RING ubiquitin ligase complex
Function	GO:0005515	protein binding
Function	GO:0031625	ubiquitin protein ligase binding
Function	GO:0004842	ubiquitin-protein transferase activity
Process	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
Process	GO:0016567	protein ubiquitination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	expressed	0.49	0.71
Schizont	expressed	0.66	0.62
Gametocyte	possibly expressed	0.46	0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0811000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -2.952 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1426500)

Relative Growth Rate: 0.31 ± 0.28
Confidence: 3.91

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1426500)

Modification: Mutated | RMgm-5338

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAU5

"Best" AlphaFill ligand hit: AN2 (amp phosphoramidate, Local RMSD=4.83) with 3IED (Global RMSD=17.44)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101330

Most Similar Human Ortholog: A0A590UJ56

TM-align score: 0.81 | RMSD: 3.79

Seq Identity: 0.20 | Length: 602 / 829

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000055130	cullin 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	13	14	24
disruptive	6	21	17	50
missense	5	20	16	42

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	30	14	41
disruptive	14	60	42	132
missense	9	42	29	106

PlasmoDB Total SNPs: 117

Non-coding: 85 | Synonymous: 26 | Nonsynonymous: 6 | Stop Codon: 0

Protein Information

Protein Length: 829 | Molecular Weight (kDa): 100.272

UniProt ID(s): Q8IAU5

PDB ID(s): None

Isoelectric Point: 5.62

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001373	Cullin, N-terminal
InterPro	IPR016158	Cullin homology domain
InterPro	IPR016159	Cullin repeat-like-containing domain superfamily
InterPro	IPR019559	Cullin protein, neddylation domain
InterPro	IPR036317	Cullin homology domain superfamily
InterPro	IPR036390	Winged helix DNA-binding domain superfamily
PFam	PF00888	Cullin, N-terminal
PFam	PF10557	Cullin protein, neddylation domain
Superfamily	SSF46785	Winged helix DNA-binding domain superfamily
Superfamily	SSF74788	Cullin repeat-like-containing domain superfamily
Superfamily	SSF75632	Cullin homology domain superfamily