PF3D7_0812100

Genome location: Pf3D7_08_v3:604,520..613,359(+)

Genome classification: Core

Function and Localization

Product Description: proteasome activator complex subunit 4, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0070577	lysine-acetylated histone binding
Function	GO:0016504	peptidase activator activity
Function	GO:0070628	proteasome binding
Process	GO:0010499	proteasomal ubiquitin-independent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.52	0.76
Trophozoite	expressed	1.40	0.36
Schizont	expressed	0.87	0.56
Gametocyte	expressed	1.10	0.46

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0812100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.509 | #Insertions: 9

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1425400)

Relative Growth Rate: 0.96 ± 0.40
Confidence: 3.23

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1425400)

Modification: Disrupted | RMgm-3860

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAV6

"Best" AlphaFill ligand hit: TEW (6-tungstotellurate(vi), Local RMSD=0.63) with 6NYS (Global RMSD=38.60)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102446

Most Similar Human Ortholog: Q14997

TM-align score: 0.75 | RMSD: 6.08

Seq Identity: 0.14 | Length: 1597 / 2651

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000068878	proteasome activator subunit 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	23	92	52	124
disruptive	56	177	105	241
missense	37	153	92	200

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	37	162	81	129
disruptive	100	319	175	392
missense	64	262	127	290

PlasmoDB Total SNPs: 296

Non-coding: 101 | Synonymous: 119 | Nonsynonymous: 76 | Stop Codon: 0

Protein Information

Protein Length: 2651 | Molecular Weight (kDa): 314.494

UniProt ID(s): Q8IAV6

PDB ID(s): None

Isoelectric Point: 8.16

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR016024	Armadillo-type fold
InterPro	IPR021843	Proteasome activator complex subunit 4 C-terminal domain
InterPro	IPR035309	Proteasome activator complex subunit 4
PFam	PF11919	Proteasome activator complex subunit 4 C-terminal domain
Superfamily	SSF48371	Armadillo-type fold