PF3D7_0812200 (HTRA2)

Genome location: Pf3D7_08_v3:614,919..617,931(-)

Genome classification: Core

Function and Localization

Product Description: serine protease HTRA2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0008233	peptidase activity
Process	GO:0007005	mitochondrion organization
Process	GO:0012501	programmed cell death
Process	GO:0006508	proteolysis
Process	GO:0010821	regulation of mitochondrion organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.01	1.00
Trophozoite	possibly expressed	0.08	0.94
Schizont	possibly expressed	0.01	0.99
Gametocyte	not expressed	0.06	0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0812200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.187 | #Insertions: 7

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1425300)

Relative Growth Rate: 1.00 ± 0.07
Confidence: 6.82

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1425300)

Modification: Disrupted | RMgm-3859

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZXD4

"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=1.49) with 5JD8 (Global RMSD=5.26)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P18584	Probable periplasmic serine endoprotease DegP-like		OrthoMCL	CHEMBL4583962CHEMBL4872003CHEMBL4879313CHEMBL5195583
O43464	Serine protease HTRA2, mitochondrial		OrthoMCL BLAST	US11149067, Compound Ahp1US11149067, Compound Ahp3US11149067, Compound Ahp4US11149067, Compound Ahp6...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100391

Most Similar Human Ortholog: A0A3B3IU24

TM-align score: 0.51 | RMSD: 3.27

Seq Identity: 0.24 | Length: 216 / 404

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000115317	HtrA serine peptidase 2
ENSG00000166033	HtrA serine peptidase 1
ENSG00000169495	HtrA serine peptidase 4
ENSG00000170801	HtrA serine peptidase 3

Protein Information

Protein Length: 404 | Molecular Weight (kDa): 46.414

UniProt ID(s): A0A143ZXD4

PDB ID(s): None

Isoelectric Point: 9.87

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001478	PDZ domain
InterPro	IPR001940	Peptidase S1C
InterPro	IPR009003	Peptidase S1, PA clan
InterPro	IPR036034	PDZ superfamily
PFam	PF13180	PDZ domain
PFam	PF13365
Superfamily	SSF50156	PDZ superfamily
Superfamily	SSF50494	Peptidase S1, PA clan

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	13	11	20
disruptive	6	22	18	55
missense	6	21	18	47

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	30	15	20
disruptive	12	62	47	84
missense	7	49	42	62

PlasmoDB Total SNPs: 150

Non-coding: 131 | Synonymous: 12 | Nonsynonymous: 7 | Stop Codon: 0