Product Description: GTP-binding protein YihA3
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.6.5.1 (Heterotrimeric G-protein GTPase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005829 | cytosol |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0043022 | ribosome binding |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | possibly expressed | 0.34 | 0.78 |
| Schizont | possibly expressed | 0.10 | 0.93 |
| Gametocyte | not expressed | 0.15 | 0.91 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0812220
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.223 | MFS: -2.666 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1306100)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZZW7
"Best" AlphaFill ligand hit: PGR (r-1,2-propanediol, Local RMSD=1.01) with 7SZS (Global RMSD=4.20)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.5.1 | heterotrimeric G-protein GTPase | GDPGpp(NH)pYM-254890phosducinpertussis toxinPasteurella multocida toxinLeu-Gly-Asn repeat-enriched proteinregulator of G protein signaling proteinbetagamma subunit of the hetreotrimeric G protein |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101142
Most Similar Human Ortholog: C9J0K3
TM-align score: 0.82 | RMSD: 2.10
Seq Identity: 0.21 | Length: 128 / 293
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000163607 | GTP binding protein 8 (putative) |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 7 | 4 | 15 |
| disruptive | 2 | 9 | 9 | 15 |
| missense | 2 | 8 | 9 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 12 | 11 | 15 |
| disruptive | 3 | 24 | 20 | 45 |
| missense | 3 | 17 | 16 | 29 |
PlasmoDB Total SNPs: 106
Non-coding: 97 | Synonymous: 7 | Nonsynonymous: 2 | Stop Codon: 0
Protein Length: 293 | Molecular Weight (kDa): 34.865
UniProt ID(s): A0A143ZZW7
PDB ID(s): None
Isoelectric Point: 10.01
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR006073 | GTP binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF01926 | GTP binding domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 32057832 | YihA GTPases localize to the apicoplast and mitochondrion of the malaria parasiteand interact with LSU of organellar ribosomes. | Gupta A, Gupta K, Habib S | 10.1016/j.molbiopara.2020.111265 |