PF3D7_0812300 (SSP3)

Genome location: Pf3D7_08_v3:618,843..620,228(-)

Genome classification: Core

Function and Localization

Product Description: sporozoite surface protein 3, putative

SignalP Peptide: MPNMLLYIFFLIFFSFYNPVKG

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0009986	cell surface
Process	GO:0071976	cell gliding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.01	0.99
Schizont	not expressed	0.08	0.95
Gametocyte	not expressed	0.11	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0812300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.336 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1425200)

Relative Growth Rate: 0.92 ± 0.18
Confidence: 4.77

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_1425200)

Modification: Tagged | RMgm-4648

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAV9

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532617

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	19	7	20
disruptive	5	25	19	47
missense	5	24	17	46

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	27	18	22
disruptive	11	55	26	83
missense	8	51	23	60

PlasmoDB Total SNPs: 29

Non-coding: 0 | Synonymous: 26 | Nonsynonymous: 3 | Stop Codon: 0

Protein Information

Protein Length: 461 | Molecular Weight (kDa): 53.755

UniProt ID(s): Q8IAV9

PDB ID(s): None

Isoelectric Point: 5.14

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
23325771	Total and putative surface proteomics of malaria parasite salivary glandsporozoites.	Lindner SE, Swearingen KE, ..., Kappe SH	10.1074/mcp.M112.024505
25156733	SSP3 is a novel Plasmodium yoelii sporozoite surface protein with a role ingliding motility.	Harupa A, Sack BK, ..., Kappe SH	10.1128/IAI.01800-14
27128092	Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics.	Swearingen KE, Lindner SE, ..., Sinnis P	10.1371/journal.ppat.1005606
38218737	Naturally acquired antibodies against Plasmodium vivax pre-erythrocytic stagevaccine antigens inhibit sporozoite invasion of human hepatocytes in vitro	Ntumngia FB, Kolli SK, ..., Adams JH	10.1038/s41598-024-51820-2