PF3D7_0812400 (KARalpha)

Genome location: Pf3D7_08_v3:621,441..624,649(-)

Genome classification: Core

Function and Localization

Product Description: karyopherin alpha

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005643	nuclear pore
Component	GO:0005634	nucleus
Function	GO:0003723	RNA binding
Function	GO:0061608	nuclear import signal receptor activity
Function	GO:0008139	nuclear localization sequence binding
Function	GO:0019904	protein domain specific binding
Function	GO:0046982	protein heterodimerization activity
Process	GO:0006607	NLS-bearing protein import into nucleus
Process	GO:0006913	nucleocytoplasmic transport
Process	GO:0009410	response to xenobiotic stimulus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.39	0.81
Trophozoite	expressed	0.86	0.53
Schizont	expressed	0.29	0.81
Gametocyte	expressed	0.74	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0812400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.153 | MFS: -2.927 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1425100)

Relative Growth Rate: 0.37 ± 0.39
Confidence: 3.30

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAW0

"Best" AlphaFill ligand hit: MLT ((2r)-2-hydroxybutanedioic acid, Local RMSD=0.33) with 5MFN (Global RMSD=2.76)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P52292	Importin subunit alpha-1	Homo sapiens	OrthoDB	MLS000073919MLS000033372MLS000034945MLS000074261...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100442

Most Similar Human Ortholog: Q5TFJ7

TM-align score: 0.91 | RMSD: 2.01

Seq Identity: 0.49 | Length: 309 / 545

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000025800	karyopherin subunit alpha 6
ENSG00000114030	karyopherin subunit alpha 1
ENSG00000182481	karyopherin subunit alpha 2
ENSG00000196911	karyopherin subunit alpha 5

Protein Information

Protein Length: 545 | Molecular Weight (kDa): 61.158

UniProt ID(s): Q7KAV0, Q8IAW0

PDB ID(s): None

Isoelectric Point: 4.93

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000225	Armadillo
InterPro	IPR002652	Importin-alpha, importin-beta-binding domain
InterPro	IPR016024	Armadillo-type fold
InterPro	IPR024931	Importin subunit alpha
InterPro	IPR032413	Atypical Arm repeat
PFam	PF00514	Armadillo
PFam	PF01749	Importin-alpha, importin-beta-binding domain
PFam	PF16186	Atypical Arm repeat
Superfamily	SSF48371	Armadillo-type fold

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	31	9	24
disruptive	0	1	1	7
missense	0	1	1	5

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	50	5	18
disruptive	1	6	6	19
missense	0	5	3	14

PlasmoDB Total SNPs: 96

Non-coding: 79 | Synonymous: 16 | Nonsynonymous: 0 | Stop Codon: 1

PMID	Title	Authors	DOI/Link
12672529	Cloning and characterization of Plasmodium falciparum homologs of nuclear importfactors, karyopherin alpha and karyopherin beta.	Mohmmed A, Kishore S, ..., Chauhan VS	10.1016/s0166-6851(02)00331-6
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
33906926	Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH	Sinha A, Baumgarten S, ..., Scherf A	10.1128/mBio.00661-21