About Gene List

PF3D7_0812600

Genome location: Pf3D7_08_v3:630,864..632,632(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin-conjugating enzyme E2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.2.23 (E2 ubiquitin-conjugating enzyme)

Curated GO (PlasmoDB):

Type GO Term Name
Function GO:0061631 ubiquitin conjugating enzyme activity
Function GO:0004842 ubiquitin-protein transferase activity
Process GO:0006281 DNA repair
Process GO:0016574 histone ubiquitination
Process GO:0043161 proteasome-mediated ubiquitin-dependent protein catabolic process
Process GO:0000209 protein polyubiquitination
Process GO:0006511 ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.07 0.97
Trophozoite expressed 0.16 0.89
Schizont expressed 0.05 0.96
Gametocyte expressed 0.44 0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0812600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS *tentative

MIS: 0.344 | MFS: -2.606 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0806000)

  • Relative Growth Rate: 0.84 ± 0.16
  • Confidence: 5.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAW2

"Best" AlphaFill ligand hit: NHE (2-[n-cyclohexylamino]ethane sulfonic acid, Local RMSD=0.62) with 4FH1 (Global RMSD=1.78)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.3.2.23 E2 ubiquitin-conjugating enzyme CC0651[1,1'-biphenyl]-2-ol[1,1'-biphenyl]-2,2'-diol4-phenyl-1,3-thiazol-2-amine[1,1'-biphenyl]-2-sulfonamide5-chloro-1,3-benzoxazole-2-thiol6-chloro-1,3-benzoxazole-2-thiol1-(1,3-benzothiazol-2-yl)methanamine2-amino-5-phenylfuran-3-carbonitrile3-(2-hydroxyphenyl)pyridin-2(1H)-one3-methyl-3,4-dihydroquinazolin-2(1H)-one5,6-dibenzo[c,e][1,2]thiazine-5,5(6H)-dione...

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
P63146 Ubiquitin-conjugating enzyme E2 B HOGENOM, OrthoDB CHEMBL3787660
P51965 Ubiquitin-conjugating enzyme E2 E1 OrthoDB US9796725, Compound I-001US9796725, Compound I-098US9796725, Compound I-003US9796725, Compound I-004...
P61088 Ubiquitin-conjugating enzyme E2 N Homo sapiens OrthoDB MLS000074439MLS000032400MLS000041097MLS000055488...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101178

Most Similar Human Ortholog: A0A0D9SG71

TM-align score: 0.98 | RMSD: 0.54

Seq Identity: 0.60 | Length: 119 / 163

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000077721 ubiquitin conjugating enzyme E2 A
ENSG00000119048 ubiquitin conjugating enzyme E2 B

Protein Information

Protein Length: 163 | Molecular Weight (kDa): 18.687

UniProt ID(s): Q8IAW2

PDB ID(s): None

Isoelectric Point: 7.24

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000608 Ubiquitin-conjugating enzyme E2
InterPro IPR016135 Ubiquitin-conjugating enzyme/RWD-like
InterPro IPR023313 Ubiquitin-conjugating enzyme, active site
PFam PF00179 Ubiquitin-conjugating enzyme E2
Superfamily SSF54495 Ubiquitin-conjugating enzyme/RWD-like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 1 14 0 11
disruptive 0 0 3 7
missense 0 0 3 6

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 3 23 5 12
disruptive 0 8 8 40
missense 0 5 6 22

PlasmoDB Total SNPs: 88

Non-coding: 86 | Synonymous: 2 | Nonsynonymous: 0 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104