PF3D7_0813500

Genome location: Pf3D7_08_v3:665,548..667,095(-)

Genome classification: Core

Function and Localization

Product Description: PUB domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.5.1.52 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.13	0.94
Trophozoite	expressed	0.54	0.68
Schizont	expressed	0.21	0.87
Gametocyte	expressed	1.33	0.39

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0813500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.811 | #Insertions: 4

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAX1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.5.1.52	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase	SDSEDTACuSO4ZnCl2FeCl3Tris-HClZ-VAD-fmkMan9GlcNAc2Triton X100triomannoseyeast mannanGlycoasparagine...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532842

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	20	13	18
disruptive	26	78	30	82
missense	24	73	30	69

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	37	13	24
disruptive	47	136	67	155
missense	37	115	49	99

PlasmoDB Total SNPs: 38

Non-coding: 0 | Synonymous: 14 | Nonsynonymous: 24 | Stop Codon: 0

Protein Information

Protein Length: 515 | Molecular Weight (kDa): 61.577

UniProt ID(s): Q8IAX1

PDB ID(s): None

Isoelectric Point: 8.94

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR018997	PUB domain
InterPro	IPR036339	PUB-like domain superfamily
PFam	PF09409	PUB domain
Superfamily	SSF143503	PUB-like domain superfamily