PF3D7_0814400

Genome location: Pf3D7_08_v3:694,720..697,978(-)

Genome classification: Core

Function and Localization

Product Description: phospholipase PLA1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.1.32 (Phospholipase A(1))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Function	GO:0004620	phospholipase activity

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.51	0.76
Trophozoite	expressed	0.24	0.86
Schizont	expressed	0.44	0.72
Gametocyte	expressed	0.43	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0814400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.122 | MFS: -2.906 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1423100)

Relative Growth Rate: 0.92 ± 0.05
Confidence: 7.28

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1423100)

Modification: Tagged | RMgm-5204

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAY0

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.1.32	phospholipase A1	KClSDSDFPEDTAPMSFMgCl2CHAPSE-600cocaineInsulinTween 20tobramycin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103749

Most Similar Human Ortholog: Q8NEL9

TM-align score: 0.47 | RMSD: 5.14

Seq Identity: 0.21 | Length: 377 / 679

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000100523	DDHD domain containing 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	6	22	11	22
disruptive	31	51	17	71
missense	19	36	14	61

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	45	19	35
disruptive	49	117	57	123
missense	26	77	50	83

PlasmoDB Total SNPs: 180

Non-coding: 137 | Synonymous: 28 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 679 | Molecular Weight (kDa): 80.761

UniProt ID(s): Q8IAY0

PDB ID(s): None

Isoelectric Point: 7.28

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004177	DDHD domain
InterPro	IPR029058	Alpha/Beta hydrolase fold
PFam	PF02862	DDHD domain
Superfamily	SSF53474	Alpha/Beta hydrolase fold