PF3D7_0815300

Genome location: Pf3D7_08_v3:721,348..725,652(+)

Genome classification: Core

Function and Localization

Product Description: FAD-dependent monooxygenase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 1.14.13.8 (Flavin-containing monooxygenase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.05	0.97
Trophozoite	expressed	0.20	0.87
Schizont	expressed	0.10	0.93
Gametocyte	expressed	0.21	0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0815300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.136 | MFS: -2.997 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAZ0

"Best" AlphaFill ligand hit: FDA (dihydroflavine-adenine dinucleotide, Local RMSD=0.81) with 4CY8 (Global RMSD=3.68)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.14.13.8	flavin-containing monooxygenase	CONOSDSKCNNADPHHgCl2MgCl2indoleThioureastearateDeprenylphosphate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_109312

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	17	55	33	63
disruptive	43	114	65	184
missense	25	94	57	153

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	26	96	48	72
disruptive	74	272	153	275
missense	42	202	121	175

PlasmoDB Total SNPs: 203

Non-coding: 59 | Synonymous: 87 | Nonsynonymous: 57 | Stop Codon: 0

Protein Information

Protein Length: 1182 | Molecular Weight (kDa): 140.226

UniProt ID(s): Q8IAZ0

PDB ID(s): None

Isoelectric Point: 9.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002938	FAD-binding domain
InterPro	IPR036188	FAD/NAD(P)-binding domain superfamily
PFam	PF01494	FAD-binding domain
Superfamily	SSF51905	FAD/NAD(P)-binding domain superfamily

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21