About Gene List

PF3D7_0815300

Genome location: Pf3D7_08_v3:721,348..725,652(+)

Genome classification: Core

Function and Localization

Product Description: FAD-dependent monooxygenase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 1.14.13.8 (Flavin-containing monooxygenase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005739 mitochondrion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring possibly expressed 0.05 0.97
Trophozoite expressed 0.20 0.87
Schizont expressed 0.10 0.93
Gametocyte expressed 0.21 0.87

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0815300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.136 | MFS: -2.997 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAZ0

"Best" AlphaFill ligand hit: FDA (dihydroflavine-adenine dinucleotide, Local RMSD=0.81) with 4CY8 (Global RMSD=3.68)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
1.14.13.8 flavin-containing monooxygenase CONOSDSKCNNADPHHgCl2MgCl2indoleThioureastearateDeprenylphosphate...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_109312

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 17 55 33 63
disruptive 43 114 65 184
missense 25 94 57 153

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 26 96 48 72
disruptive 74 272 153 275
missense 42 202 121 175

PlasmoDB Total SNPs: 203

Non-coding: 59 | Synonymous: 87 | Nonsynonymous: 57 | Stop Codon: 0

Protein Information

Protein Length: 1182 | Molecular Weight (kDa): 140.226

UniProt ID(s): Q8IAZ0

PDB ID(s): None

Isoelectric Point: 9.33

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002938 FAD-binding domain
InterPro IPR036188 FAD/NAD(P)-binding domain superfamily
PFam PF01494 FAD-binding domain
Superfamily SSF51905 FAD/NAD(P)-binding domain superfamily

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
34494883 A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. van Esveld SL, Meerstein-Kessel L, ..., Huynen MA 10.1128/mSphere.00614-21