PF3D7_0815700 (Ub)

Genome location: Pf3D7_08_v3:732,891..735,068(-)

Genome classification: Core

Function and Localization

Product Description: ubiquitin

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Function	GO:0016887	ATP hydrolysis activity
Process	GO:0010564	regulation of cell cycle process
Process	GO:0030433	ubiquitin-dependent ERAD pathway

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.98
Trophozoite	possibly expressed	0.20	0.87
Schizont	not expressed	0.07	0.95
Gametocyte	not expressed	0.16	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0815700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.124 | MFS: -3.107 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0715100)

Relative Growth Rate: 0.26 ± 0.14
Confidence: 5.36

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0715100)

Modification: Disrupted | RMgm-2248

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4U7

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532635

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	16	12	19
disruptive	3	34	20	62
missense	3	33	18	52

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	32	14	23
disruptive	9	90	52	114
missense	8	69	45	55

PlasmoDB Total SNPs: 100

Non-coding: 73 | Synonymous: 19 | Nonsynonymous: 8 | Stop Codon: 0

Protein Information

Protein Length: 373 | Molecular Weight (kDa): 44.914

UniProt ID(s): C0H4U7

PDB ID(s): None

Isoelectric Point: 10.1

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000626	Ubiquitin-like domain
InterPro	IPR029071	Ubiquitin-like domain superfamily
PFam	PF00240	Ubiquitin-like domain
Superfamily	SSF54236	Ubiquitin-like domain superfamily

PMID	Title	Authors	DOI/Link
19502583	An unusual ERAD-like complex is targeted to the apicoplast of Plasmodiumfalciparum.	Spork S, Hiss JA, ..., Przyborski JM	10.1128/EC.00083-09
30212465	Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites.	Boucher MJ, Ghosh S, ..., Yeh E	10.1371/journal.pbio.2005895
32817449	Redesigned Tet R-Aptamer System To Control Gene Expression in Plasmodiumfalciparum	Rajaram K, Liu HB, Prigge ST	10.1128/mSphere.00457-20