Product Description: male gamete fusion factor HAP2P
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005929 | cilium |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.12 | 0.94 |
| Trophozoite | expressed | 0.17 | 0.89 |
| Schizont | possibly expressed | 0.04 | 0.98 |
| Gametocyte | expressed | 0.41 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0816300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.224 | MFS: -2.717 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0714500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0714500)
Modification: Disrupted | RMgm-2243
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4U8
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=3.98) with 7NPE (Global RMSD=23.46)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 44 | 24 | 56 |
| disruptive | 23 | 89 | 70 | 144 |
| missense | 17 | 84 | 66 | 129 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 81 | 35 | 73 |
| disruptive | 43 | 212 | 122 | 248 |
| missense | 30 | 180 | 105 | 198 |
PlasmoDB Total SNPs: 198
Non-coding: 109 | Synonymous: 61 | Nonsynonymous: 28 | Stop Codon: 0
Protein Length: 999 | Molecular Weight (kDa): 122.377
UniProt ID(s): C0H4U8
PDB ID(s): None
Isoelectric Point: 9.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR040326 | HAP2/GCS1 |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16278450 | Assembly of the Hap2p/Hap3p/Hap4p/Hap5p-DNA complex in Saccharomyces cerevisiae | McNabb DS, Pinto I | 10.1128/EC.4.11.1829-1839.2005 |
| 7900416 | A genetic screen to isolate genes regulated by the yeast CCAAT-box bindingprotein Hap2p | Dang VD, Valens M, Bolotin-Fukuhara M, Daignan-Fornier B | 10.1002/yea.320101004 |
| 8951815 | Cloning of the ASN1 and ASN2 genes encoding asparagine synthetases inSaccharomyces cerevisiae: differential regulation by the CCAAT-box-bindingfactor | Dang VD, Valens M, Bolotin-Fukuhara M, Daignan-Fornier B | 10.1046/j.1365-2958.1996.d01-1715.x |
| 9372932 | The Saccharomyces cerevisiae Hap5p homolog from fission yeast reveals twoconserved domains that are essential for assembly of heterotetramericCCAAT-binding factor | McNabb DS, Tseng KA, Guarente L | 10.1128/MCB.17.12.7008 |
| 30803782 | Identification of metabolic engineering targets for improving glycerolassimilation ability of Saccharomyces cerevisiae based on adaptive laboratoryevolution and transcriptome analysis | Kawai K, Kanesaki Y, Yoshikawa H, Hirasawa T | 10.1016/j.jbiosc.2019.02.001 |
| 12393187 | The yeast gene COQ5 is differentially regulated by Mig1p, Rtg3p and Hap2p | Hagerman RA, Willis RA | 10.1016/s0167-4781(02)00496-7 |
| 16098534 | The CCAAT-binding complex of eukaryotes: evolution of a second NLS in the HapBsubunit of the filamentous fungus Aspergillus nidulans despite functionalconservation at the molecular level between yeast, A.nidulans and human | Tuncher A, Sprote P, Gehrke A, Brakhage AA | 10.1016/j.jmb.2005.06.068 |