PF3D7_0816400

Genome location: Pf3D7_08_v3:756,978..758,683(+)

Genome classification: Core

Function and Localization

Product Description: EF-hand calcium-binding domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0005509	calcium ion binding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	possibly expressed	0.03	0.98
Trophozoite	expressed	0.27	0.83
Schizont	expressed	0.43	0.73
Gametocyte	expressed	0.57	0.67

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0816400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.705 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0714400)

Relative Growth Rate: 0.97 ± 0.06
Confidence: 7.04

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0714400)

Modification: Disrupted | RMgm-2242

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4U9

"Best" AlphaFill ligand hit: RET (retinal, Local RMSD=2.13) with 6MKV (Global RMSD=16.68)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_156219

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	8	8	14
disruptive	1	4	5	16
missense	1	4	5	14

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	20	10	14
disruptive	3	13	18	47
missense	2	10	14	33

PlasmoDB Total SNPs: 50

Non-coding: 46 | Synonymous: 3 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 233 | Molecular Weight (kDa): 28.102

UniProt ID(s): C0H4U9

PDB ID(s): None

Isoelectric Point: 6.05

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002048	EF-hand domain
InterPro	IPR011992	EF-hand domain pair
InterPro	IPR018247	EF-Hand 1, calcium-binding site
PFam	PF13202	EF-hand domain
Superfamily	SSF47473	EF-hand domain pair

PMID	Title	Authors	DOI/Link
30204084	Elucidating the mitochondrial proteome of Toxoplasma gondii reveals the presenceof a divergent cytochrome c oxidase	Seidi A, Muellner-Wong LS, ..., van Dooren GG	10.7554/eLife.38131
34494883	A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology.	van Esveld SL, Meerstein-Kessel L, ..., Huynen MA	10.1128/mSphere.00614-21