Product Description: chaperone protein ClpB1
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0009368 | endopeptidase Clp complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0016887 | ATP hydrolysis activity |
| Process | GO:0034605 | cellular response to heat |
| Process | GO:0006986 | response to unfolded protein |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.16 | 0.92 |
| Trophozoite | expressed | 0.86 | 0.55 |
| Schizont | expressed | 0.12 | 0.93 |
| Gametocyte | expressed | 0.60 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0816600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.165 | MFS: -2.809 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0931200)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IB03
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.56) with 4CIU (Global RMSD=6.64)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100223
Most Similar Human Ortholog: A0A2R8Y602
TM-align score: 0.64 | RMSD: 4.07
Seq Identity: 0.30 | Length: 283 / 1070
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000162129 | caseinolytic mitochondrial matrix peptidase chaperone subunit B |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 42 | 32 | 55 |
| disruptive | 10 | 52 | 21 | 47 |
| missense | 9 | 49 | 19 | 44 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 24 | 101 | 44 | 68 |
| disruptive | 23 | 92 | 40 | 142 |
| missense | 17 | 74 | 34 | 81 |
PlasmoDB Total SNPs: 136
Non-coding: 98 | Synonymous: 29 | Nonsynonymous: 9 | Stop Codon: 0
Protein Length: 1070 | Molecular Weight (kDa): 122.508
Isoelectric Point: 8.89
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001270 | ClpA/B family |
| InterPro | IPR003593 | AAA+ ATPase domain |
| InterPro | IPR003959 | ATPase, AAA-type, core |
| InterPro | IPR004176 | Clp, repeat (R) domain |
| InterPro | IPR018368 | ClpA/B, conserved site 1 |
| InterPro | IPR019489 | Clp ATPase, C-terminal |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR036628 | Clp, N-terminal domain superfamily |
| InterPro | IPR041546 | ClpA/ClpB, AAA lid domain |
| PFam | PF00004 | ATPase, AAA-type, core |
| PFam | PF02861 | Clp, repeat (R) domain |
| PFam | PF07724 | ATPase, AAA-type, core |
| PFam | PF10431 | Clp ATPase, C-terminal |
| PFam | PF17871 | ClpA/ClpB, AAA lid domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| Superfamily | SSF81923 | Clp, N-terminal domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 20887733 | The Clp chaperones and proteases of the human malaria parasite Plasmodiumfalciparum. | El Bakkouri M, Pow A, ..., Houry WA | 10.1016/j.jmb.2010.09.051 |
| 23910910 | Role of calcineurin and actin dynamics in regulated secretion of micronemeproteins in Plasmodium falciparum merozoites during erythrocyte invasion. | Singh S, More KR, Chitnis CE | 10.1111/cmi.12177 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 32484795 | A replication-competent late liver stage-attenuated human malaria parasite | Goswami D, Betz W, ..., Kappe SH | 10.1172/jci.insight.135589 |
| 11005383 | The Escherichia coli heat shock protein ClpB restores acquired thermotolerance toa cyanobacterial clpB deletion mutant | Eriksson MJ, Clarke AK | 10.1379/1466-1268(2000)005<0255:techsp>2.0.co;2 |
| 26130467 | Structural mapping of the ClpB ATPases of Plasmodium falciparum: Targeting protein folding and secretion for antimalarial drug design | AhYoung AP, Koehl A, Cascio D, Egea PF | 10.1002/pro.2739 |
| 37552754 | Knock-sideways by inducible ER retrieval enables a unique approach for studying Plasmodium-secreted proteins | Fierro MA, Hussain T, Campin LJ, Beck JR | 10.1073/pnas.2308676120 |