PF3D7_0816900 (AK2)

Genome location: Pf3D7_08_v3:770,704..771,789(+)

Genome classification: Core

Function and Localization

Product Description: adenylate kinase 2

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.4.3 (Adenylate kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005739	mitochondrion
Component	GO:0005886	plasma membrane
Component	GO:0020005	symbiont-containing vacuole membrane
Function	GO:0004017	adenylate kinase activity
Function	GO:0005515	protein binding
Process	GO:0009132	nucleoside diphosphate metabolic process
Process	GO:0006163	purine nucleotide metabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.01	0.99
Trophozoite	not expressed	0.02	0.99
Schizont	not expressed	0.01	1.00
Gametocyte	not expressed	0.29	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0816900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.141 | MFS: -3.054 | #Insertions: 0

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0713900)

Relative Growth Rate: 1.08 ± 0.13
Confidence: 5.46

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0713900)

Modification: Disrupted | RMgm-2238

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB06

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.49) with 3TLX (Global RMSD=5.69)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.4.3	adenylate kinase	BRENDA SOAP query unsuccessful

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P29410	Adenylate kinase 2, mitochondrial		OrthoDB	CHEMBL605639CHEMBL604399CHEMBL605639CHEMBL604399

Orthology Information

Ortholog Group (OrthoMCL): OG6_143002

No human ortholog(s)

Protein Information

Protein Length: 275 | Molecular Weight (kDa): 32.497

UniProt ID(s): Q14EL6, Q8IB06

PDB ID(s): None

Isoelectric Point: 7.54

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000850	Adenylate kinase/UMP-CMP kinase
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00406	N/A
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	5	6	19
disruptive	8	27	11	40
missense	7	27	10	33

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	21	11	17
disruptive	19	61	19	61
missense	11	53	17	47

PlasmoDB Total SNPs: 34

Non-coding: 23 | Synonymous: 3 | Nonsynonymous: 7 | Stop Codon: 1

PMID	Title	Authors	DOI/Link
18973776	Myristoylated adenylate kinase-2 of Plasmodium falciparum forms a heterodimerwith myristoyltransferase.	Rahlfs S, Koncarevic S, ..., Becker K	https://pubmed.ncbi.nlm.nih.gov/18973776/
22819813	Subcellular localization of adenylate kinases in Plasmodium falciparum.	Ma J, Rahlfs S, ..., Becker K	10.1016/j.febslet.2012.07.013
25909331	Alternative Protein Secretion in the Malaria Parasite Plasmodium falciparum.	Thavayogarajah T, Gangopadhyay P, ..., Holder AA	10.1371/journal.pone.0125191