PF3D7_0817100

Genome location: Pf3D7_08_v3:774,134..777,131(+)

Genome classification: Core

Function and Localization

Product Description: tRNA modification GTPase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 3.6.5.1 (Heterotrimeric G-protein GTPase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005886	plasma membrane
Function	GO:0003924	GTPase activity
Function	GO:0015093	ferrous iron transmembrane transporter activity
Process	GO:0002098	tRNA wobble uridine modification

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.07	0.96
Trophozoite	no data	0.22	0.87
Schizont	no data	0.04	0.97
Gametocyte	no data	0.40	0.78

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0817100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.128 | MFS: -2.835 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4V2

"Best" AlphaFill ligand hit: FON (n-{[4-({[(6r)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-l-glutamic acid, Local RMSD=0.04) with 1XZQ (Global RMSD=7.03)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.5.1	heterotrimeric G-protein GTPase	GDPGpp(NH)pYM-254890phosducinpertussis toxinPasteurella multocida toxinLeu-Gly-Asn repeat-enriched proteinregulator of G protein signaling proteinbetagamma subunit of the hetreotrimeric G protein

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101467

Most Similar Human Ortholog: Q969Y2

TM-align score: 0.82 | RMSD: 3.30

Seq Identity: 0.24 | Length: 452 / 830

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000130299	GTP binding protein 3, mitochondrial

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	48	26	56
disruptive	16	84	60	150
missense	11	70	53	123

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	97	36	41
disruptive	37	202	110	187
missense	22	145	94	146

PlasmoDB Total SNPs: 111

Non-coding: 43 | Synonymous: 49 | Nonsynonymous: 19 | Stop Codon: 0

Protein Information

Protein Length: 830 | Molecular Weight (kDa): 97.942

UniProt ID(s): C0H4V2

PDB ID(s): None

Isoelectric Point: 9.96

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006073	GTP binding domain
InterPro	IPR018948	GTP-binding protein TrmE, N-terminal
InterPro	IPR025867	MnmE, helical domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF01926	GTP binding domain
PFam	PF10396	GTP-binding protein TrmE, N-terminal
PFam	PF12631	MnmE, helical domain
Superfamily	SSF103025	P-loop containing nucleoside triphosphate hydrolase
Superfamily	SSF116878
Superfamily	SSF52540