Genome location: Pf3D7_08_v3:827,788..832,382(+)
Genome classification: Core
Product Description: 14-3-3 protein
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0042393 | histone binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.48 | 0.76 |
| Trophozoite | expressed | 0.69 | 0.61 |
| Schizont | expressed | 0.34 | 0.78 |
| Gametocyte | expressed | 0.43 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0818200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.585 | MFS: -3.082 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0712600)
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0712600)
Modification: Tagged | RMgm-1492
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4V6
"Best" AlphaFill ligand hit: TME (propane, Local RMSD=0.07) with 4ZDR (Global RMSD=1.43)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P61981 | 14-3-3 protein gamma | Homo sapiens | HOGENOM, OrthoDB | MLS000077269MLS000077184DAUNORUBICINAdriblastina... |
| P63104 | 14-3-3 protein zeta/delta | OrthoDB | CHEMBL4069746CHEMBL4096939CHEMBL4068123CHEMBL4096939... | |
| P31947 | 14-3-3 protein sigma | Homo sapiens | OrthoDB | CHEMBL3813927CHEMBL4243744CHEMBL4250552CHEMBL4237531... |
Ortholog Group (OrthoMCL): OG6_100283
Most Similar Human Ortholog: K7EM20
TM-align score: 0.99 | RMSD: 0.50
Seq Identity: 0.68 | Length: 115 / 262
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000108953 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon |
| ENSG00000164924 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta |
| ENSG00000166913 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta |
| ENSG00000274474 | tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon |
Protein Length: 262 | Molecular Weight (kDa): 30.193
PDB ID(s): None
Isoelectric Point: 4.56
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000308 | 14-3-3 protein |
| InterPro | IPR023409 | 14-3-3 protein, conserved site |
| InterPro | IPR023410 | 14-3-3 domain |
| InterPro | IPR036815 | 14-3-3 domain superfamily |
| PFam | PF00244 | 14-3-3 domain |
| Superfamily | SSF48445 | 14-3-3 domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 362
Non-coding: 357 | Synonymous: 5 | Nonsynonymous: 0 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 23308157 | Comprehensive histone phosphorylation analysis and identification of Pf14-3-3protein as a histone H3 phosphorylation reader in malaria parasites. | Dastidar EG, Dzeyk K, ..., Lopez-Rubio JJ | 10.1371/journal.pone.0053179 |
| 27129434 | Characterization of an A-kinase anchoring protein-like suggests an alternativeway of PKA anchoring in Plasmodium falciparum. | Bandje K, Naissant B, ..., Merckx A | 10.1186/s12936-016-1275-9 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 32484216 | Molecular dynamics simulations and biochemical characterization of Pf14-3-3 andPfCDPK1 interaction towards its role in growth of human malaria parasite. | Jain R, Dey P, ..., Singh S | 10.1042/BCJ20200145 |
| 32817103 | Phosphorylation-Dependent Assembly of a 14-3-3 Mediated Signaling Complex during Red Blood Cell Invasion by Plasmodium falciparum Merozoites | More KR, Kaur I, ..., Chitnis CE | 10.1128/mBio.01287-20 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 23457405 | Trypanosoma brucei 14-3-3I and II proteins predominantly form a heterodimerstructure that acts as a potent cell cycle regulator in vivo | Inoue M, Yasuda K, ..., Horikoshi N | 10.1093/jb/mvt016 |
| 21203569 | Phosphorylation-dependent protein interaction with Trypanosoma brucei 14-3-3proteins that display atypical target recognition | Inoue M, Yasuda K, ..., Fukuma T | 10.1371/journal.pone.0015566 |