PF3D7_0818700

Genome location: Pf3D7_08_v3:847,541..854,736(-)

Genome classification: Core

Function and Localization

Product Description: DNA helicase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.12 (DNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0008094	ATP-dependent activity, acting on DNA
Function	GO:0003677	DNA binding
Function	GO:0003682	chromatin binding
Process	GO:0043044	ATP-dependent chromatin remodeling

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.84	0.62
Trophozoite	possibly expressed	1.25	0.40
Schizont	possibly expressed	0.55	0.72
Gametocyte	not expressed	1.04	0.50

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0818700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.629 | #Insertions: 7

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0712100)

Relative Growth Rate: 0.79 ± 0.35
Confidence: 3.50

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0712100)

Modification: Disrupted | RMgm-2226

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4V8

"Best" AlphaFill ligand hit: KH2 (3-(1-methylpiperidinium-1-yl)propane-1-sulfonate, Local RMSD=0.16) with 5HZR (Global RMSD=1.40)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.12	DNA helicase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103309

Most Similar Human Ortholog: Q9H4L7

TM-align score: 0.53 | RMSD: 4.04

Seq Identity: 0.34 | Length: 601 / 1223

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000163104	SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	56	44	70
disruptive	35	141	90	227
missense	23	121	73	183

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	20	118	60	89
disruptive	83	347	195	387
missense	51	268	142	252

PlasmoDB Total SNPs: 328

Non-coding: 237 | Synonymous: 49 | Nonsynonymous: 42 | Stop Codon: 0

Protein Information

Protein Length: 1223 | Molecular Weight (kDa): 143.663

UniProt ID(s): C0H4V8

PDB ID(s): None

Isoelectric Point: 6.27

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000330	SNF2-related, N-terminal domain
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00176	SNF2-related, N-terminal domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase