Product Description: heat shock protein 70
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0020020 | food vacuole |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0016887 | ATP hydrolysis activity |
| Function | GO:0003723 | RNA binding |
| Function | GO:0031072 | heat shock protein binding |
| Function | GO:0051787 | misfolded protein binding |
| Function | GO:0032266 | phosphatidylinositol-3-phosphate binding |
| Function | GO:0005515 | protein binding |
| Function | GO:0044183 | protein folding chaperone |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0034620 | cellular response to unfolded protein |
| Process | GO:0051085 | chaperone cofactor-dependent protein refolding |
| Process | GO:0042026 | protein refolding |
| Process | GO:0009408 | response to heat |
| Process | GO:0006986 | response to unfolded protein |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 3.85 | 0.05 |
| Trophozoite | expressed | 4.42 | 0.00 |
| Schizont | expressed | 2.48 | 0.24 |
| Gametocyte | expressed | 3.26 | 0.05 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0818900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.225 | MFS: -2.666 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0711900)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IB24
"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.08) with 4JN4 (Global RMSD=3.06)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P11021 | Endoplasmic reticulum chaperone BiP | Homo sapiens | HOGENOM | CHEMBL3576921Hexach-loropheneHexach-loropheneadenosine-derived inhibitor, 12... |
| P54652 | Heat shock-related 70 kDa protein 2 | HOGENOM | CHEMBL4473449CHEMBL4519613CHEMBL4545529CHEMBL4545529... | |
| P11142 | Heat shock cognate 71 kDa protein | Homo sapiens | HOGENOM, OMA | CHEMBL258670cid_274127cid_274125Aenosine 5'-Diphosphate |
Ortholog Group (OrthoMCL): OG6_100083
Most Similar Human Ortholog: E9PK54
TM-align score: 0.99 | RMSD: 0.88
Seq Identity: 0.77 | Length: 183 / 677
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000044574 | heat shock protein family A (Hsp70) member 5 |
| ENSG00000109971 | heat shock protein family A (Hsp70) member 8 |
| ENSG00000126803 | heat shock protein family A (Hsp70) member 2 |
| ENSG00000173110 | heat shock protein family A (Hsp70) member 6 |
| ENSG00000204388 | heat shock protein family A (Hsp70) member 1B |
| ENSG00000204389 | heat shock protein family A (Hsp70) member 1A |
| ENSG00000204390 | heat shock protein family A (Hsp70) member 1 like |
| ENSG00000206383 | heat shock protein family A (Hsp70) member 1 like |
| ENSG00000212866 | heat shock protein family A (Hsp70) member 1B |
| ENSG00000215328 | heat shock protein family A (Hsp70) member 1A |
| ENSG00000224501 | heat shock protein family A (Hsp70) member 1B |
| ENSG00000226704 | heat shock protein family A (Hsp70) member 1 like |
| ENSG00000231555 | heat shock protein family A (Hsp70) member 1B |
| ENSG00000232804 | heat shock protein family A (Hsp70) member 1B |
| ENSG00000234258 | heat shock protein family A (Hsp70) member 1 like |
| ENSG00000234475 | heat shock protein family A (Hsp70) member 1A |
| ENSG00000235941 | heat shock protein family A (Hsp70) member 1A |
| ENSG00000236251 | heat shock protein family A (Hsp70) member 1 like |
| ENSG00000237724 | heat shock protein family A (Hsp70) member 1A |
Protein Length: 677 | Molecular Weight (kDa): 73.915
UniProt ID(s): P11144, Q25791, Q25962, Q8IB24
PDB ID(s): None
Isoelectric Point: 5.33
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR013126 | Heat shock protein 70 family |
| InterPro | IPR018181 | Heat shock protein 70, conserved site |
| InterPro | IPR029047 | Heat shock protein 70kD, peptide-binding domain superfamily |
| InterPro | IPR029048 | Heat shock protein 70kD, C-terminal domain superfamily |
| InterPro | IPR043129 | ATPase, nucleotide binding domain |
| PFam | PF00012 | Heat shock protein 70 family |
| Superfamily | SSF100920 | Heat shock protein 70kD, peptide-binding domain superfamily |
| Superfamily | SSF100934 | Heat shock protein 70kD, C-terminal domain superfamily |
| Superfamily | SSF53067 | ATPase, nucleotide binding domain |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 37 | 15 | 43 |
| disruptive | 6 | 15 | 4 | 32 |
| missense | 1 | 4 | 0 | 14 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 73 | 22 | 23 |
| disruptive | 12 | 27 | 18 | 61 |
| missense | 2 | 7 | 10 | 33 |
PlasmoDB Total SNPs: 99
Non-coding: 76 | Synonymous: 19 | Nonsynonymous: 4 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 18674634 | The Plasmodium falciparum heat shock protein 40, Pfj4, associates with heat shockprotein 70 and shows similar heat induction and localisation patterns. | Pesce ER, Acharya P, ..., Blatch GL | 10.1016/j.biocel.2008.06.011 |
| 21136929 | Food vacuole proteome of the malarial parasite Plasmodium falciparum. | Lamarque M, Tastet C, ..., Dubremetz JF | 10.1002/prca.200700112 |
| 21191678 | Plasmodium falciparum encodes a single cytosolic type I Hsp40 that functionallyinteracts with Hsp70 and is upregulated by heat shock. | Botha M, Chiang AN, ..., Blatch GL | 10.1007/s12192-010-0250-6 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 24494818 | A secretory multifunctional serine protease, DegP of Plasmodium falciparum, playsan important role in thermo-oxidative stress, parasite growth and development. | Sharma S, Jadli M, ..., Malhotra P | 10.1111/febs.12732 |
| 26083397 | Overexpression, Purification and Characterisation of the Plasmodium falciparumHsp70-z (PfHsp70-z) Protein. | Zininga T, Achilonu I, ..., Shonhai A | 10.1371/journal.pone.0129445 |
| 26267894 | Plasmodium falciparum Hop (PfHop) Interacts with the Hsp70 Chaperone in aNucleotide-Dependent Fashion and Exhibits Ligand Selectivity. | Zininga T, Makumire S, ..., Shonhai A | 10.1371/journal.pone.0135326 |
| 26317863 | Characterization of Precursor PfHsp60 in Plasmodium falciparum Cytosol during ItsAsexual Development in Human Erythrocytes. | Padma Priya P, Grover M, Tatu US, Natarajan V | 10.1371/journal.pone.0136401 |
| 26894764 | Plasmodium falciparum Hsp70-z, an Hsp110 homologue, exhibits independentchaperone activity and interacts with Hsp70-1 in a nucleotide-dependent fashion. | Zininga T, Achilonu I, ..., Shonhai A | 10.1007/s12192-016-0678-4 |
| 26988354 | Analysis of the interactome of the Ser/Thr Protein Phosphatase type 1 inPlasmodium falciparum. | Hollin T, De Witte C, ..., Khalife J | 10.1186/s12864-016-2571-z |
| 27714937 | PlasmoSEP: Predicting surface-exposed proteins on the malaria parasite usingsemisupervised self-training and expert-annotated data. | El-Manzalawy Y, Munoz EE, Lindner SE, Honavar V | 10.1002/pmic.201600249 |
| 28944300 | Proteomic analysis of extracellular vesicles from a Plasmodium falciparum Kenyanclinical isolate defines a core parasite secretome. | Abdi A, Yu L, ..., Rayner J | 10.12688/wellcomeopenres.11910.2 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 30682336 | Proteomic analysis of Plasmodium falciparum histone deacetylase 1 complexproteins. | Engel JA, Norris EL, ..., Andrews KT | 10.1016/j.exppara.2019.01.008 |
| 32512819 | Comparative Characterization of Plasmodium falciparum Hsp70-1 Relative to E | Lebepe CM, Matambanadzo PR, ..., Shonhai A | 10.3390/biom10060856 |
| 32975513 | Phosphatidylinositol 3-phosphate and Hsp70 protect Plasmodium falciparum fromheat-induced cell death | Lu KY, Pasaje CFA, ..., Derbyshire E | 10.7554/eLife.56773 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 38418371 | Plasmodium falciparum J-dot localized J domain protein A8iJp modulates thechaperone activity of human HSPA8 | Sahu W, Bai T, ..., Reddy KS | 10.1002/1873-3468.14836 |