PF3D7_0819000

Genome location: Pf3D7_08_v3:863,419..868,820(-)

Genome classification: Core

Function and Localization

Product Description: ribonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005847	mRNA cleavage and polyadenylation specificity factor complex
Function	GO:0003723	RNA binding
Process	GO:0006398	mRNA 3'-end processing by stem-loop binding and cleavage
Process	GO:0006378	mRNA polyadenylation
Process	GO:0098789	pre-mRNA cleavage required for polyadenylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	1.13	0.52
Trophozoite	expressed	0.78	0.58
Schizont	expressed	0.23	0.86
Gametocyte	expressed	0.80	0.58

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0819000

Resistome Missense Mutations: N346Y

Resistome Compounds with Missense Mutations: AN10248

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.313 | MFS: -2.655 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0711800)

Relative Growth Rate: 0.06 ± 0.04
Confidence: 7.76

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB25

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532484

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	60	40	59
disruptive	71	220	100	264
missense	31	160	74	211

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	20	114	38	87
disruptive	124	353	156	317
missense	53	276	127	248

PlasmoDB Total SNPs: 251

Non-coding: 119 | Synonymous: 75 | Nonsynonymous: 57 | Stop Codon: 0

Protein Information

Protein Length: 1313 | Molecular Weight (kDa): 157.676

UniProt ID(s): Q8IB25

PDB ID(s): None

Isoelectric Point: 4.71

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001279	Metallo-beta-lactamase
InterPro	IPR022712	Beta-Casp domain
InterPro	IPR027075	Cleavage and polyadenylation specificity factor subunit 2
InterPro	IPR036866	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like
PFam	PF16661	Metallo-beta-lactamase
Superfamily	SSF56281	Ribonuclease Z/Hydroxyacylglutathione hydrolase-like