PF3D7_0819200 (PLP5)

Genome location: Pf3D7_08_v3:871,410..873,440(+)

Genome classification: Core

Function and Localization

Product Description: perforin-like protein 5

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.13	0.93
Trophozoite	not expressed	0.11	0.94
Schizont	not expressed	0.02	0.99
Gametocyte	not expressed	0.05	0.97

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0819200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.141 | MFS: -2.919 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0711600)

Modification: Disrupted | RMgm-123

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A5K1K8C6

"Best" AlphaFill ligand hit: NDP (nadph dihydro-nicotinamide-adenine-dinucleotide phosphate, Local RMSD=9.05) with 1J3K (Global RMSD=18.56)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533768

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	35	19	40
disruptive	5	49	19	73
missense	5	47	16	70

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	70	30	42
disruptive	18	95	48	118
missense	13	83	41	95

PlasmoDB Total SNPs: 33

Non-coding: 0 | Synonymous: 22 | Nonsynonymous: 9 | Stop Codon: 2

Protein Information

Protein Length: 676 | Molecular Weight (kDa): 79.405

UniProt ID(s): A0A5K1K8C6

PDB ID(s): None

Isoelectric Point: 9.05

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR020864	Membrane attack complex component/perforin (MACPF) domain
PFam	PF01823	Membrane attack complex component/perforin (MACPF) domain
Superfamily	N/A	N/A