About Gene List

PF3D7_0819400 (PLP4)

Genome location: Pf3D7_08_v3:878,495..880,459(+)

Genome classification: Core

Function and Localization

Product Description: perforin-like protein 4

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0045177 apical part of cell
Component GO:0071944 cell periphery
Component GO:0031982 vesicle
Process GO:0044409 entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.02 0.99
Trophozoite not expressed 0.01 0.99
Schizont possibly expressed 0.01 1.00
Gametocyte possibly expressed 0.07 0.96

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0819400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.145 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0711400)

Modification: Tagged | RMgm-4462

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB29

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533561

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 8 38 18 55
disruptive 8 58 34 92
missense 8 57 33 86

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 13 73 35 55
disruptive 20 136 83 190
missense 17 119 68 141

PlasmoDB Total SNPs: 36

Non-coding: 0 | Synonymous: 27 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 654 | Molecular Weight (kDa): 76.347

UniProt ID(s): Q8IB29

PDB ID(s): None

Isoelectric Point: 8.48

Protein Domain Annotations:

Source Family ID Description
InterPro IPR020864 Membrane attack complex component/perforin (MACPF) domain
PFam PF01823 Membrane attack complex component/perforin (MACPF) domain
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
26166358 Perforin-like protein PPLP4 is crucial for mosquito midgut infection byPlasmodium falciparum. Wirth CC, Bennink S, ..., Pradel G 10.1016/j.molbiopara.2015.06.005
30102727 Essential role of Plasmodium perforin-like protein 4 in ookinete midgut passage. Deligianni E, Silmon de Monerri NC, ..., Siden-Kiamos I 10.1371/journal.pone.0201651