PF3D7_0820200 (PGPS)

Genome location: Pf3D7_08_v3:911,627..915,386(-)

Genome classification: Core

Function and Localization

Product Description: phosphatidylglycerophosphate synthase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.8.5 (CDP-diacylglycerol--glycerol-3-phosphate 1-phosphatidyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0008444	CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity
Process	GO:0032049	cardiolipin biosynthetic process
Process	GO:0006072	glycerol-3-phosphate metabolic process
Process	GO:0006655	phosphatidylglycerol biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.58	0.72
Trophozoite	expressed	0.75	0.58
Schizont	expressed	0.51	0.69
Gametocyte	expressed	0.40	0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0820200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.146 | MFS: -2.739 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB37

"Best" AlphaFill ligand hit: AZM (5-acetamido-1,3,4-thiadiazole-2-sulfonamide, Local RMSD=0.87) with 3W6H (Global RMSD=18.50)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.8.5	CDP-diacylglycerol-glycerol-3-phosphate 1-phosphatidyltransferase	InositolTriton X-100LiponucleotideCDP-diacylglycerolThioreactive agentsglycerol 3-phosphate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102888

Most Similar Human Ortholog: Q32NB8

TM-align score: 0.77 | RMSD: 2.18

Seq Identity: 0.28 | Length: 452 / 661

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000087157	phosphatidylglycerophosphate synthase 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	37	25	37
disruptive	28	103	57	102
missense	23	80	50	86

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	79	24	44
disruptive	58	206	78	190
missense	39	160	64	120

PlasmoDB Total SNPs: 267

Non-coding: 207 | Synonymous: 31 | Nonsynonymous: 28 | Stop Codon: 1

Protein Information

Protein Length: 661 | Molecular Weight (kDa): 78.063

UniProt ID(s): Q8IB37, Q9BMH1

PDB ID(s): None

Isoelectric Point: 7.86

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR016270	CDP-alcohol phosphatidyltransferase class-II family
InterPro	IPR025202	Phospholipase D-like domain
PFam	PF13091	Phospholipase D-like domain
Superfamily	SSF56024	N/A