PF3D7_0821300 (DHX36)

Genome location: Pf3D7_08_v3:958,332..962,154(-)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent RNA helicase DHX36, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005681	spliceosomal complex
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Process	GO:0000398	mRNA splicing, via spliceosome

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.73	0.65
Trophozoite	not expressed	0.49	0.72
Schizont	not expressed	0.31	0.83
Gametocyte	possibly expressed	0.66	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0821300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.123 | MFS: -2.945 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0709500)

Relative Growth Rate: 0.15 ± 0.17
Confidence: 4.96

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB47

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.10) with 6L5M (Global RMSD=4.40)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_151105

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	59	32	57
disruptive	19	100	61	154
missense	16	92	57	138

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	112	41	70
disruptive	50	218	85	222
missense	31	189	73	168

PlasmoDB Total SNPs: 189

Non-coding: 134 | Synonymous: 29 | Nonsynonymous: 26 | Stop Codon: 0

Protein Information

Protein Length: 867 | Molecular Weight (kDa): 101.8

UniProt ID(s): Q8IB47

PDB ID(s): None

Isoelectric Point: 9.38

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR007502	Helicase-associated domain
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR011709	Domain of unknown function DUF1605
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF04408	Helicase-associated domain
PFam	PF07717	Domain of unknown function DUF1605
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase