Product Description: polyribonucleotide 5'-hydroxyl-kinase Clp1, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.1.78 (Polynucleotide 5'-hydroxyl-kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
| Component | GO:0000214 | tRNA-intron endonuclease complex |
| Function | GO:0051733 | polydeoxyribonucleotide kinase activity |
| Function | GO:0051731 | polynucleotide 5'-hydroxyl-kinase activity |
| Process | GO:0006396 | RNA processing |
| Process | GO:0006378 | mRNA polyadenylation |
| Process | GO:0006388 | tRNA splicing, via endonucleolytic cleavage and ligation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.78 | 0.64 |
| Trophozoite | expressed | 0.58 | 0.66 |
| Schizont | possibly expressed | 0.12 | 0.93 |
| Gametocyte | expressed | 0.48 | 0.72 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0821600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.192 | MFS: -2.738 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IB50
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=1.14) with 4OHX (Global RMSD=2.47)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.1.78 | polynucleotide 5'-hydroxyl-kinase | ADPGTPCTPUTPKClEDTANH4+dATPdTTPdCTPdGTPAgNO3... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101503
Most Similar Human Ortholog: Q92989
TM-align score: 0.86 | RMSD: 3.12
Seq Identity: 0.23 | Length: 408 / 674
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000172409 | cleavage factor polyribonucleotide kinase subunit 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 35 | 19 | 44 |
| disruptive | 24 | 85 | 56 | 99 |
| missense | 15 | 72 | 52 | 78 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 18 | 70 | 34 | 47 |
| disruptive | 45 | 186 | 95 | 171 |
| missense | 26 | 148 | 75 | 117 |
PlasmoDB Total SNPs: 110
Non-coding: 39 | Synonymous: 45 | Nonsynonymous: 26 | Stop Codon: 0
Protein Length: 674 | Molecular Weight (kDa): 79.061
UniProt ID(s): Q8IB50
PDB ID(s): None
Isoelectric Point: 7.29
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR032319 | Polyribonucleotide 5'-hydroxyl-kinase Clp1, P-loop domain |
| InterPro | IPR032324 | Clp1, N-terminal beta-sandwich domain |
| PFam | PF16573 | Clp1, N-terminal beta-sandwich domain |
| PFam | PF16575 | Polyribonucleotide 5'-hydroxyl-kinase Clp1, P-loop domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 37674027 | Fission yeast Cdc14-like phosphatase Flp1/Clp1 modulates the transcriptional response to oxidative stress | Canete JA, Andres S, ..., Sacristan MP | 10.1038/s41598-023-41869-w |
| 37606665 | Systematic Analysis of Diverse Polynucleotide Kinase Clp1 Family Proteins inEukaryotes: Three Unique Clp1 Proteins of Trypanosoma brucei | Saito M, Inose R, ..., Kanai A | 10.1007/s00239-023-10128-x |
| 35923846 | Uncoupling of Mitosis and Cytokinesis Upon a Prolonged Arrest in Metaphase IsInfluenced by Protein Phosphatases and Mitotic Transcription in Fission Yeast | Chica N, Portantier M, ..., Lopez-Aviles S | 10.3389/fcell.2022.876810 |