Genome location: Pf3D7_08_v3:1,012,878..1,017,485(-)
Genome classification: Core
Product Description: serine/threonine protein kinase VPS15, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:1903561 | extracellular vesicle |
| Component | GO:0005770 | late endosome |
| Component | GO:0071561 | nucleus-vacuole junction |
| Component | GO:0034271 | phosphatidylinositol 3-kinase complex, class III, type I |
| Component | GO:0034272 | phosphatidylinositol 3-kinase complex, class III, type II |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0030242 | autophagy of peroxisome |
| Process | GO:0045324 | late endosome to vacuole transport |
| Process | GO:0016236 | macroautophagy |
| Process | GO:0036092 | phosphatidylinositol-3-phosphate biosynthetic process |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0006623 | protein targeting to vacuole |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.15 | 0.92 |
| Trophozoite | expressed | 0.31 | 0.81 |
| Schizont | expressed | 0.18 | 0.87 |
| Gametocyte | possibly expressed | 0.45 | 0.74 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0823000
Resistome Missense Mutations: N742Y, N830K
Resistome Compounds with Missense Mutations: BI-2536, MMV085203
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.137 | MFS: -2.942 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0707600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IB64
"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.13) with 4EQM (Global RMSD=6.77)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 12 | 58 | 48 | 79 |
| disruptive | 78 | 227 | 114 | 277 |
| missense | 37 | 173 | 92 | 226 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 119 | 64 | 101 |
| disruptive | 152 | 465 | 189 | 391 |
| missense | 82 | 346 | 146 | 249 |
PlasmoDB Total SNPs: 259
Non-coding: 88 | Synonymous: 99 | Nonsynonymous: 72 | Stop Codon: 0
Protein Length: 1332 | Molecular Weight (kDa): 162.313
UniProt ID(s): Q8IB64
PDB ID(s): None
Isoelectric Point: 9.37
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR016024 | Armadillo-type fold |
| PFam | N/A | N/A |
| Superfamily | SSF48371 | Armadillo-type fold |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 33608523 | 20S proteasomes secreted by the malaria parasite promote its growth. | Dekel E, Yaffe D, ..., Regev-Rudzki N | 10.1038/s41467-021-21344-8 |
| 36318587 | A VPS15-like kinase regulates apicoplast biogenesis and autophagy by promotingPI3P generation in Toxoplasma gondii | Rawat RS, Bansal P, Sharma P | 10.1371/journal.ppat.1010922 |