Genome location: Pf3D7_08_v3:1,039,724..1,041,357(-)
Genome classification: Core
Product Description: lipoate-protein ligase B
SignalP Peptide: MNKINLLVLISLTFIILLNP
# Transmembrane Domains: 0
EC Numbers: 2.3.1.181 (Lipoyl(octanoyl) transferase);6.3.1.20 (Lipoate--protein ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0016979 | lipoate-protein ligase activity |
| Process | GO:0009107 | lipoate biosynthetic process |
| Process | GO:0009249 | protein lipoylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.04 | 0.98 |
| Trophozoite | not expressed | 0.06 | 0.96 |
| Schizont | not expressed | 0.01 | 0.99 |
| Gametocyte | not expressed | 0.04 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0823600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.194 | #Insertions: 5
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0707000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0707000)
Modification: Disrupted | RMgm-2201
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IB70
"Best" AlphaFill ligand hit: LPA (lipoic acid, Local RMSD=7.00) with 5T8U (Global RMSD=12.33)
BRENDA EC Inhibitors:
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101396
Most Similar Human Ortholog: A6NK58
TM-align score: 0.86 | RMSD: 2.74
Seq Identity: 0.18 | Length: 227 / 434
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000175536 | lipoyl(octanoyl) transferase 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 38 | 8 | 24 |
| disruptive | 7 | 53 | 31 | 80 |
| missense | 7 | 52 | 30 | 80 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 53 | 14 | 30 |
| disruptive | 19 | 128 | 49 | 103 |
| missense | 14 | 116 | 41 | 79 |
PlasmoDB Total SNPs: 57
Non-coding: 31 | Synonymous: 17 | Nonsynonymous: 9 | Stop Codon: 0
Protein Length: 434 | Molecular Weight (kDa): 51.638
UniProt ID(s): Q8IB70
PDB ID(s): None
Isoelectric Point: 10.1
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR020605 | Octanoyltransferase, conserved site |
| PFam | N/A | N/A |
| Superfamily | SSF55681 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 15225307 | The human malaria parasite Plasmodium falciparum has distinct organelle-specificlipoylation pathways. | Wrenger C, Muller S | 10.1111/j.1365-2958.2004.04112.x |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 33713652 | Plasmodium falciparum LipB mutants display altered redox and carbon metabolism inasexual stages and cannot complete sporogony in Anopheles mosquitoes | Biddau M, Santha Kumar TR, ..., Sheiner L | 10.1016/j.ijpara.2020.10.011 |