PF3D7_0824100

Genome location: Pf3D7_08_v3:1,052,541..1,054,493(+)

Genome classification: Core

Function and Localization

Product Description: 3'-5' exonuclease, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.11.1 (Exodeoxyribonuclease I);3.1.11.2 (Exodeoxyribonuclease III)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.09	0.96
Trophozoite	expressed	0.16	0.91
Schizont	expressed	0.05	0.97
Gametocyte	possibly expressed	0.27	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0824100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.121 | MFS: -3.001 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0706500)

Relative Growth Rate: 0.12 ± 0.11
Confidence: 5.88

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB75

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.11.1	exodeoxyribonuclease I	NTPEDTAcamptothecinendonuclease CtIPfullerene-oligonucleotide conjugatesingle-stranded DNA-binding protein5'-thiophosphorylated oligonucleotide
3.1.11.2	exodeoxyribonuclease III	DNAPNAEDTAZnCl2p-chloromercuribenzoate

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_151531

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	18	7	27
disruptive	18	60	33	89
missense	18	59	32	80

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	35	16	22
disruptive	32	140	49	120
missense	28	125	44	81

PlasmoDB Total SNPs: 62

Non-coding: 25 | Synonymous: 26 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 406 | Molecular Weight (kDa): 48.101

UniProt ID(s): Q8IB75

PDB ID(s): None

Isoelectric Point: 8.98

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002562	3'-5' exonuclease domain
InterPro	IPR012337	Ribonuclease H-like superfamily
PFam	PF01612	3'-5' exonuclease domain
Superfamily	SSF53098	Ribonuclease H-like superfamily