Genome location: Pf3D7_08_v3:1,112,930..1,140,853(-)
Genome classification: Core
Product Description: HECT-like E3 ubiquitin ligase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 2
EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Function | GO:0061630 | ubiquitin protein ligase activity |
| Function | GO:0004842 | ubiquitin-protein transferase activity |
| Process | GO:0016049 | cell growth |
| Process | GO:0045732 | positive regulation of protein catabolic process |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
| Process | GO:0000209 | protein polyubiquitination |
| Process | GO:0016567 | protein ubiquitination |
| Process | GO:0006511 | ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 2.34 | 0.21 |
| Trophozoite | expressed | 2.79 | 0.07 |
| Schizont | expressed | 1.74 | 0.32 |
| Gametocyte | expressed | 2.61 | 0.10 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0826100
Resistome Missense Mutations: S703F, S1398Y
Resistome Compounds with Missense Mutations: BI-2536, MMV019066
Resistome # Samples with Disruptive Mutations: 2 (2 missense, 2 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -2.928 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0704500)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.3.2.19 | ubiquitin-protein ligase | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101783
Most Similar Human Ortholog: Not available
TM-align score: 0.00 | RMSD: 0.00
Seq Identity: 0.00 | Length: / 8591
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000086758 | HECT, UBA and WWE domain containing E3 ubiquitin protein ligase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 90 | 529 | 347 | 684 |
| disruptive | 371 | 1405 | 728 | 1760 |
| missense | 205 | 1011 | 586 | 1361 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 186 | 1156 | 433 | 638 |
| disruptive | 720 | 2852 | 1166 | 2463 |
| missense | 386 | 2091 | 895 | 1635 |
PlasmoDB Total SNPs: 1108
Non-coding: 138 | Synonymous: 555 | Nonsynonymous: 415 | Stop Codon: 0
Protein Length: 8591 | Molecular Weight (kDa): 1005.144
UniProt ID(s): Q8IB94
PDB ID(s): None
Isoelectric Point: 4.93
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000569 | HECT domain |
| InterPro | IPR035983 | HECT, E3 ligase catalytic domain |
| PFam | PF00632 | HECT domain |
| Superfamily | SSF56204 | HECT, E3 ligase catalytic domain |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 28790316 | A Plasmodium yoelii HECT-like E3 ubiquitin ligase regulates parasite growth andvirulence. | Nair SC, Xu R, ..., Su XZ | 10.1038/s41467-017-00267-3 |