PF3D7_0826400 (CPT)

Genome location: Pf3D7_08_v3:1,148,010..1,149,830(-)

Genome classification: Core

Function and Localization

Product Description: cis-prenyltransferase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.5.1.- (Transferring alkyl or aryl groups, other than methyl groups.);2.5.1.31 (Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific))

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Function	GO:0002094	polyprenyltransferase activity
Function	GO:0004659	prenyltransferase activity
Process	GO:0019408	dolichol biosynthetic process
Process	GO:0016094	polyprenol biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	not expressed	0.13	0.91
Schizont	not expressed	0.18	0.86
Gametocyte	not expressed	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0826400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.173 | MFS: -3.257 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IB97

"Best" AlphaFill ligand hit: B29 ([2-(3-dibenzo[b,d]furan-4-ylphenyl)-1-hydroxyethane-1,1-diyl]bis(phosphonic acid), Local RMSD=0.02) with 2E98 (Global RMSD=2.72)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.5.1.-	Ex: N1-aminopropylagmatine synthase	APHBPYS04070YS05035taxodoneJDS05119JDS05120celastroltaxodionearenaroneMMV019313bacitracinclodronate...
2.5.1.31	ditrans,polycis-undecaprenyl-diphosphate synthase [(2E,6E)-farnesyl-diphosphate specific]	EDTANH4+SO42-BPH-629targocil2-propanolclomiphenediphosphateiodoacetateButanedioneMAC-0557874MAC-0547630...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q97SR4	Isoprenyl transferase	Streptococcus pneumoniae	OMA	CHEMBL256483CHEMBL271482CHEMBL272913CHEMBL272696...
P60472	Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific)		OMA, OrthoMCL	CHEMBL5182237

Orthology Information

Ortholog Group (OrthoMCL): OG6_100493

Most Similar Human Ortholog: Q5T0A3

TM-align score: 0.85 | RMSD: 2.41

Seq Identity: 0.34 | Length: 206 / 317

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000117682	dehydrodolichyl diphosphate synthase subunit

Protein Information

Protein Length: 317 | Molecular Weight (kDa): 38.014

UniProt ID(s): Q8IB97

PDB ID(s): None

Isoelectric Point: 7.14

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001441	Decaprenyl diphosphate synthase-like
InterPro	IPR036424	Decaprenyl diphosphate synthase-like superfamily
PFam	PF01255	Decaprenyl diphosphate synthase-like
Superfamily	SSF64005	Decaprenyl diphosphate synthase-like superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	18	15	25
disruptive	10	41	19	41
missense	8	39	19	39

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	36	19	22
disruptive	15	75	23	58
missense	13	67	22	48

PlasmoDB Total SNPs: 126

Non-coding: 99 | Synonymous: 20 | Nonsynonymous: 7 | Stop Codon: 0