PF3D7_0826500 (UBE4B)

Genome location: Pf3D7_08_v3:1,151,361..1,157,299(+)

Genome classification: Core

Function and Localization

Product Description: ubiquitin conjugation factor E4 B, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.3.2.19 (Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0034450	ubiquitin-ubiquitin ligase activity
Process	GO:0000209	protein polyubiquitination
Process	GO:0030433	ubiquitin-dependent ERAD pathway

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.34	0.84
Trophozoite	expressed	0.76	0.59
Schizont	expressed	0.51	0.70
Gametocyte	expressed	0.80	0.57

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0826500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.905 | MFS: -2.783 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0704100)

Relative Growth Rate: 0.51 ± 0.17
Confidence: 4.92

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0704100)

Modification: Disrupted | RMgm-2181

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4Y0

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.3.2.19	ubiquitin-protein ligase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101833

Most Similar Human Ortholog: O95155

TM-align score: 0.63 | RMSD: 3.64

Seq Identity: 0.20 | Length: 892 / 1326

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000130939	ubiquitination factor E4B

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	121	42	99
disruptive	32	138	66	178
missense	20	123	57	155

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	37	201	60	103
disruptive	52	287	113	258
missense	34	235	93	169

PlasmoDB Total SNPs: 304

Non-coding: 186 | Synonymous: 76 | Nonsynonymous: 42 | Stop Codon: 0

Protein Information

Protein Length: 1326 | Molecular Weight (kDa): 154.634

UniProt ID(s): C0H4Y0

PDB ID(s): None

Isoelectric Point: 5.97

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003613	U box domain
InterPro	IPR019474	Ubiquitin conjugation factor E4, core
PFam	PF04564	U box domain
PFam	PF10408	Ubiquitin conjugation factor E4, core
Superfamily	SSF57850	N/A