Genome location: Pf3D7_08_v3:1,172,102..1,177,112(+)
Genome classification: Core
Product Description: translation initiation factor IF-2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0005525 | GTP binding |
| Function | GO:0003924 | GTPase activity |
| Function | GO:0003743 | translation initiation factor activity |
| Process | GO:0006413 | translational initiation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.14 | 0.93 |
| Trophozoite | expressed | 0.63 | 0.64 |
| Schizont | expressed | 0.30 | 0.81 |
| Gametocyte | expressed | 0.51 | 0.71 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0827100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.122 | MFS: -2.932 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1231400)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBA3
"Best" AlphaFill ligand hit: 7PG (2,5,8,11,14,17,20,23-octaoxapentacosan-25-ol, Local RMSD=0.13) with 4RJL (Global RMSD=4.69)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100987
Most Similar Human Ortholog: C9JIY5
TM-align score: 0.89 | RMSD: 1.23
Seq Identity: 0.04 | Length: 26 / 1397
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000085760 | mitochondrial translational initiation factor 2 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 21 | 81 | 52 | 106 |
| disruptive | 68 | 229 | 122 | 329 |
| missense | 34 | 185 | 104 | 269 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 38 | 159 | 83 | 105 |
| disruptive | 114 | 529 | 193 | 437 |
| missense | 54 | 431 | 150 | 291 |
PlasmoDB Total SNPs: 219
Non-coding: 81 | Synonymous: 68 | Nonsynonymous: 70 | Stop Codon: 0
Protein Length: 1397 | Molecular Weight (kDa): 164.182
UniProt ID(s): Q8IBA3
PDB ID(s): None
Isoelectric Point: 8.94
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000795 | Transcription factor, GTP-binding domain |
| InterPro | IPR005225 | Small GTP-binding protein domain |
| InterPro | IPR009000 | Translation protein, beta-barrel domain superfamily |
| InterPro | IPR015760 | Translation initiation factor IF- 2 |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| InterPro | IPR036925 | Translation initiation factor IF-2, domain 3 superfamily |
| PFam | PF00009 | Transcription factor, GTP-binding domain |
| Superfamily | SSF50447 | Translation protein, beta-barrel domain superfamily |
| Superfamily | SSF52156 | Translation initiation factor IF-2, domain 3 superfamily |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25689481 | Targeting and function of proteins mediating translation initiation in organellesof Plasmodium falciparum. | Haider A, Allen SM, ..., Habib S | 10.1111/mmi.12972 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |