Genome location: Pf3D7_08_v3:1,210,692..1,211,860(+)
Genome classification: Core
Product Description: rhomboid protease ROM3
SignalP Peptide: N/A
# Transmembrane Domains: 8
EC Numbers: 3.4.21.- (Serine endopeptidases.);3.4.21.105 (Rhomboid protease)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0008233 | peptidase activity |
| Process | GO:0006465 | signal peptide processing |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.00 | 1.00 |
| Trophozoite | expressed | 0.01 | 0.99 |
| Schizont | expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.42 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0828000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.146 | MFS: -3.446 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0933500)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0702700)
Modification: Disrupted | RMgm-5063
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A5K1K8S0
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.21.- | Ex: physarolisin | SDSCysDFPGAWSTIDANE64KD1EDTAPMSFDMSOTLCK... |
| 3.4.21.105 | rhomboid protease | dodecyl maltosidedichloroisocoumarin3,4-dichloroisocoumarinn-nonyl-beta-D-glucosidediisopropyl fluorophosphonate3,4-dichloro-1H-2-benzopyran-1-one7-amino-4-chloro-3-methoxyisocoumarin3-butyl-4-(pent-4-yn-1-yl)oxetan-2-one1-myristoyl-sn-glycero-3-phosphocholine1,2-dihexanoyl-sn-glycero-3-phosphocholine1,2-dimyristoyl-sn-glycero-3-phosphocholinephenyl 2-oxo-4-phenylazetidine-1-carboxylate... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| Q9WZ53 | Rhomboid domain-containing protein | OrthoMCL BLAST | cid_1609 | |
| P54493 | Rhomboid protease GluP | OrthoMCL BLAST | cid_1609CHEMBL4225584CHEMBL4225103CHEMBL79597... | |
| O29251 | AN1-type domain-containing protein | OrthoMCL BLAST | cid_1609(4S)‐4‐(pent‐4‐yn‐1‐yl)‐3‐(3,4,5‐trimethoxyphenyl)oxetan‐2‐one (45) |
Ortholog Group (OrthoMCL): OG6_100562
Most Similar Human Ortholog: Q96CC6
TM-align score: 0.73 | RMSD: 3.90
Seq Identity: 0.26 | Length: 239 / 267
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000007384 | rhomboid 5 homolog 1 |
| ENSG00000129667 | rhomboid 5 homolog 2 |
Protein Length: 267 | Molecular Weight (kDa): 30.685
UniProt ID(s): A0A5K1K8S0
PDB ID(s): None
Isoelectric Point: 8.46
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002610 | Peptidase S54, rhomboid |
| InterPro | IPR022764 | Peptidase S54, rhomboid domain |
| PFam | PF01694 | Peptidase S54, rhomboid domain |
| Superfamily | SSF144091 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 21 | 8 | 25 |
| disruptive | 5 | 17 | 13 | 24 |
| missense | 5 | 17 | 12 | 21 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 39 | 14 | 20 |
| disruptive | 8 | 38 | 17 | 57 |
| missense | 7 | 33 | 17 | 44 |
PlasmoDB Total SNPs: 54
Non-coding: 41 | Synonymous: 10 | Nonsynonymous: 3 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 17040128 | Two Plasmodium rhomboid proteases preferentially cleave different adhesinsimplicated in all invasive stages of malaria. | Baker RP, Wijetilaka R, Urban S | 10.1371/journal.ppat.0020113 |