PF3D7_0828200

Genome location: Pf3D7_08_v3:1,215,932..1,221,181(+)

Genome classification: Core

Function and Localization

Product Description: leucine--tRNA ligase, putative

SignalP Peptide: MFFKFVRFLIYIFLYSLCYQHVNNVFA

# Transmembrane Domains: 4

EC Numbers: 6.1.1.4 (Leucine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Component	GO:0005739	mitochondrion
Function	GO:0004823	leucine-tRNA ligase activity
Process	GO:0006429	leucyl-tRNA aminoacylation
Process	GO:0032543	mitochondrial translation
Process	GO:0006412	translation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.22	0.90
Trophozoite	expressed	1.10	0.45
Schizont	expressed	0.52	0.71
Gametocyte	expressed	0.94	0.52

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0828200

Resistome Missense Mutations: R848K

Resistome Compounds with Missense Mutations: GNF-Pf-5611

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.125 | MFS: -2.982 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0702500)

Relative Growth Rate: 0.22 ± 0.43
Confidence: 3.10

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBB3

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.15) with 7NU6 (Global RMSD=3.87)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.4	leucine-tRNA ligase	ATPADPAMPNEMDTTITPDTNB2'-dATPadenosinenorvalinenovobiocinBC-LI-0186...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P9WFV1	Leucine--tRNA ligase		OrthoMCL	CHEMBL4081755CHEMBL4077894CHEMBL4075879CHEMBL4066786...
P07813	Leucine--tRNA ligase		OrthoMCL	CHEMBL4163450CHEMBL4159778CHEMBL4171058CHEMBL4160841...

Orthology Information

Ortholog Group (OrthoMCL): OG6_101393

Most Similar Human Ortholog: Q15031

TM-align score: 0.73 | RMSD: 4.80

Seq Identity: 0.27 | Length: 759 / 1481

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000011376	leucyl-tRNA synthetase 2, mitochondrial

Protein Information

Protein Length: 1481 | Molecular Weight (kDa): 178.43

UniProt ID(s): Q8IBB3

PDB ID(s): None

Isoelectric Point: 9.99

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001412	Aminoacyl-tRNA synthetase, class I, conserved site
InterPro	IPR002300	Aminoacyl-tRNA synthetase, class Ia
InterPro	IPR002302	Leucine-tRNA ligase
InterPro	IPR009080	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
InterPro	IPR013155	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
InterPro	IPR025709	Leucyl-tRNA synthetase, editing domain
PFam	PF00133	Aminoacyl-tRNA synthetase, class Ia
PFam	PF08264	Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
PFam	PF13603	Leucyl-tRNA synthetase, editing domain
Superfamily	SSF47323	Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
Superfamily	SSF52374

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	86	40	115
disruptive	37	190	124	250
missense	26	176	117	225

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	28	159	71	112
disruptive	79	415	180	368
missense	52	360	155	274

PlasmoDB Total SNPs: 136

Non-coding: 37 | Synonymous: 48 | Nonsynonymous: 51 | Stop Codon: 0