PF3D7_0828500

Genome location: Pf3D7_08_v3:1,226,140..1,228,476(-)

Genome classification: Core

Function and Localization

Product Description: translation initiation factor eIF-2B subunit alpha, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.3.1.23 (S-methyl-5-thioribose-1-phosphate isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005851	eukaryotic translation initiation factor 2B complex
Function	GO:0003743	translation initiation factor activity
Process	GO:0006446	regulation of translational initiation
Process	GO:0006413	translational initiation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	not expressed	0.06	0.97
Trophozoite	expressed	0.53	0.69
Schizont	expressed	0.46	0.72
Gametocyte	expressed	0.53	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0828500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.135 | MFS: -2.843 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0702200)

Relative Growth Rate: 0.50 ± 0.08
Confidence: 6.46

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0702200)

Modification: Disrupted | RMgm-2169

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBB6

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.3.1.23	S-methyl-5-thioribose-1-phosphate isomerase	RNAi

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102141

Most Similar Human Ortholog: Q14232

TM-align score: 0.80 | RMSD: 2.93

Seq Identity: 0.24 | Length: 281 / 342

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000111361	eukaryotic translation initiation factor 2B subunit alpha

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	21	7	20
disruptive	6	23	17	29
missense	6	22	17	28

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	6	44	9	16
disruptive	9	65	27	65
missense	9	57	21	47

PlasmoDB Total SNPs: 160

Non-coding: 148 | Synonymous: 8 | Nonsynonymous: 4 | Stop Codon: 0

Protein Information

Protein Length: 342 | Molecular Weight (kDa): 40.027

UniProt ID(s): Q8IBB6

PDB ID(s): None

Isoelectric Point: 6.41

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000649	Initiation factor 2B-related
InterPro	IPR037171	NagB/RpiA transferase-like
PFam	PF01008	Initiation factor 2B-related
Superfamily	SSF100950	NagB/RpiA transferase-like