Genome location: Pf3D7_08_v3:1,237,184..1,241,229(-)
Genome classification: Core
Product Description: GPI-anchored micronemal antigen
SignalP Peptide: MKYYTSLYVALIIALCQAVSA
# Transmembrane Domains: 2
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0046658 | anchored component of plasma membrane |
| Component | GO:0009986 | cell surface |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0031244 | extrinsic component of cell outer membrane |
| Component | GO:0020009 | microneme |
| Component | GO:0044310 | osmiophilic body |
| Function | GO:0046812 | host cell surface binding |
| Function | GO:0046789 | host cell surface receptor binding |
| Process | GO:0044409 | entry into host |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | not expressed | 0.57 | 0.82 |
| Trophozoite | not expressed | 0.05 | 0.97 |
| Schizont | expressed | 0.42 | 0.85 |
| Gametocyte | possibly expressed | 0.31 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0828800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.183 | MFS: -3.301 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0701900)
Modification: Mutated | RMgm-5191
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBB9
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 35 | 21 | 57 |
| disruptive | 31 | 69 | 26 | 96 |
| missense | 24 | 48 | 21 | 77 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 13 | 90 | 30 | 62 |
| disruptive | 53 | 150 | 84 | 215 |
| missense | 33 | 107 | 64 | 134 |
PlasmoDB Total SNPs: 222
Non-coding: 117 | Synonymous: 67 | Nonsynonymous: 38 | Stop Codon: 0
Protein Length: 738 | Molecular Weight (kDa): 85.25
UniProt ID(s): A0A193PDA0, A0A193PDB6, A0A193PDC0, A0A193PDT7, A0A193PDT9, A0A193PDU2, A0A193PDV0, A0A193PDV2, A0A193PEN0, A0A193PEN5, Q8IBB9
PDB ID(s): None
Isoelectric Point: 6.07
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | N/A | N/A |
| PFam | N/A | N/A |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 19435743 | ESG: extended similarity group method for automated protein function prediction. | Chitale M, Hawkins T, Park C, Kihara D | 10.1093/bioinformatics/btp309 |
| 19820120 | Novel putative glycosylphosphatidylinositol-anchored micronemal antigen ofPlasmodium falciparum that binds to erythrocytes. | Hinds L, Green JL, ..., Holder AA | 10.1128/EC.00218-09 |
| 26292257 | Discovery of Novel Plasmodium falciparum Pre-Erythrocytic Antigens for VaccineDevelopment. | Aguiar JC, Bolton J, ..., Richie TL | 10.1371/journal.pone.0136109 |
| 27128092 | Interrogating the Plasmodium Sporozoite Surface: Identification ofSurface-Exposed Proteins and Demonstration of Glycosylation on CSP and TRAP byMass Spectrometry-Based Proteomics. | Swearingen KE, Lindner SE, ..., Sinnis P | 10.1371/journal.ppat.1005606 |
| 27432909 | Comparative Proteomics and Functional Analysis Reveal a Role of Plasmodiumfalciparum Osmiophilic Bodies in Malaria Parasite Transmission. | Suarez-Cortes P, Sharma V, ..., Alano P | 10.1074/mcp.M116.060681 |
| 35202655 | Glycosylphosphatidylinositol-anchored micronemal antigen (GAMA) interacts withthe band 3 receptor to promote erythrocyte invasion by malaria parasites. | Lu J, Chu R, ..., Cheng Y | 10.1016/j.jbc.2022.101765 |