PF3D7_0829400

Genome location: Pf3D7_08_v3:1,257,111..1,260,032(-)

Genome classification: Core

Function and Localization

Product Description: prolyl 4-hydroxylase subunit alpha, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 1.14.11.2 (Procollagen-proline 4-dioxygenase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005783	endoplasmic reticulum
Process	GO:0018401	peptidyl-proline hydroxylation to 4-hydroxy-L-proline

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	expressed	0.14	0.91
Schizont	expressed	0.27	0.81
Gametocyte	expressed	0.19	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0829400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: 0.223 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0701300)

Relative Growth Rate: 0.97 ± 0.08
Confidence: 6.37

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0701300)

Modification: Disrupted | RMgm-2164

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4Z0

"Best" AlphaFill ligand hit: AKG (2-oxoglutaric acid, Local RMSD=0.33) with 5HV0 (Global RMSD=9.94)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
1.14.11.2	procollagen-proline 4-dioxygenase	CO2EDTAEGTADMOGCuCl2CoCl2ZnSO4NiCl2MgSO4MnSO4malateHOE077...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_112288

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	27	15	49
disruptive	8	38	26	65
missense	7	38	23	61

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	5	67	23	40
disruptive	17	98	55	129
missense	10	87	47	84

PlasmoDB Total SNPs: 189

Non-coding: 171 | Synonymous: 11 | Nonsynonymous: 7 | Stop Codon: 0

Protein Information

Protein Length: 441 | Molecular Weight (kDa): 51.689

UniProt ID(s): C0H4Z0

PDB ID(s): None

Isoelectric Point: 6.24

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006620	Prolyl 4-hydroxylase, alpha subunit
PFam	N/A	N/A
Superfamily	N/A	N/A