Genome location: Pf3D7_08_v3:1,358,188..1,363,639(+)
Genome classification: Core
Product Description: cytoadherence linked asexual protein 8
SignalP Peptide: MISYFKVVVFIIFFFLFLKRRVIC
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB): None
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.24 | 0.88 |
| Trophozoite | no data | 0.33 | 0.81 |
| Schizont | no data | 2.33 | 0.20 |
| Gametocyte | no data | 0.09 | 0.95 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0831600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.705 | #Insertions: 4
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1400600)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZVM1
"Best" AlphaFill ligand hit: DTP (, Local RMSD=2.65) with 5IM3 (Global RMSD=20.13)
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 25 | 26 | 5 | 31 |
| disruptive | 109 | 53 | 29 | 98 |
| missense | 70 | 43 | 25 | 79 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 31 | 36 | 18 | 48 |
| disruptive | 131 | 93 | 79 | 178 |
| missense | 77 | 75 | 63 | 124 |
PlasmoDB Total SNPs: 275
Non-coding: 134 | Synonymous: 63 | Nonsynonymous: 78 | Stop Codon: 0
Protein Length: 1394 | Molecular Weight (kDa): 165.613
UniProt ID(s): A0A143ZVM1, B0M0V6, B0M154, B0M161, Q4AE84
PDB ID(s): None
Isoelectric Point: 8.63
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR005553 | Cytoadherence-linked asexual protein |
| PFam | PF03805 | Cytoadherence-linked asexual protein |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 18155305 | Diversity and evolution of the rhoph1/clag multigene family of Plasmodiumfalciparum | Iriko H, Kaneko O, ..., Torii M | 10.1016/j.molbiopara.2007.11.004 |
| 23264726 | Stable Allele Frequency Distribution of the Plasmodium falciparum clag GenesEncoding Components of the High Molecular Weight Rhoptry Protein Complex | Alexandre JS, Xangsayarath P, ..., Kaneko O | 10.2149/tmh.2012-13 |
| 22028613 | Positive selection on the Plasmodium falciparum clag2 gene encoding a componentof the erythrocyte-binding rhoptry protein complex | Alexandre JS, Kaewthamasorn M, ..., Kaneko O | 10.2149/tmh.2011-12 |