Genome location: Pf3D7_08_v3:1,373,212..1,376,988(-)
Genome classification: SubtelomericHypervariable
Product Description: histidine-rich protein II
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0020020 | food vacuole |
| Component | GO:0030430 | host cell cytoplasm |
| Component | GO:0033644 | host cell membrane |
| Process | GO:0042167 | heme catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 1.91 | 0.35 |
| Trophozoite | no data | 0.42 | 0.82 |
| Schizont | no data | 0.22 | 0.88 |
| Gametocyte | no data | 0.14 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0831800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -1.914 | #Insertions: 1
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: A0A143ZVM5
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P05227 | Histidine-rich protein PFHRP-II | Plasmodium falciparum | OrthoMCL BLAST | CHEMBL152635CHEMBL2111198CHEMBL152052CHEMBL346177... |
Protein Length: 305 | Molecular Weight (kDa): 32.409
UniProt ID(s): A0A143ZVM5
PDB ID(s): None
Isoelectric Point: 7.02
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR008779 | Plasmodium histidine-rich |
| PFam | PF05403 | Plasmodium histidine-rich |
| Superfamily | N/A | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 340
Non-coding: 96 | Synonymous: 155 | Nonsynonymous: 89 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 3533951 | Secretion of a malarial histidine-rich protein (Pf HRP II) from Plasmodiumfalciparum-infected erythrocytes. | Howard RJ, Uni S, ..., Taylor DW | 10.1083/jcb.103.4.1269 |
| 8539625 | Plasmodium hemozoin formation mediated by histidine-rich proteins. | Sullivan DJ Jr, Gluzman IY, Goldberg DE | 10.1126/science.271.5246.219 |
| 21511958 | Inhibition of antithrombin by Plasmodium falciparum histidine-rich protein II. | Ndonwi M, Burlingame OO, ..., Goldberg DE | 10.1182/blood-2010-12-326876 |
| 24077522 | Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodiumfalciparum parasites from Peru. | Akinyi S, Hayden T, ..., Udhayakumar V | 10.1038/srep02797 |
| 24472178 | Analysis of pfhrp2 genetic diversity in Senegal and implications for use of rapiddiagnostic tests. | Deme AB, Park DJ, ..., Volkman SK | 10.1186/1475-2875-13-34 |
| 24521645 | Detection of histidine rich protein & lactate dehydrogenase of Plasmodiumfalciparum in malaria patients by sandwich ELISA using in-house reagents. | Verma P, Biswas S, ..., Rao DN | https://pubmed.ncbi.nlm.nih.gov/24521645/ |
| 27273825 | Plasmodium falciparum Histidine-Rich Protein I I Compromises Brain Endothelial Barriers and May Promote Cerebral Malaria Pathogenesis | Pal P, Daniels BP, ..., Goldberg DE | 10.1128/mBio.00617-16 |
| 31186056 | Genetic diversity of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) andits effect on the performance of PfHRP2-based rapid diagnostic tests. | Mussa A, Talib M, Mohamed Z, Hajissa K | 10.1186/s13104-019-4361-6 |
| 31631203 | A network-based approach reveals novel invasion and Maurer's clefts-relatedproteins in Plasmodium falciparum. | Das D, Krishnan SR, Roy A, Bulusu G | 10.1039/c9mo00124g |
| 33926477 | HRP2 and HRP3 cross-reactivity and implications for HRP2-based RDT use in regionswith Plasmodium falciparum hrp2 gene deletions. | Kong A, Wilson SA, ..., Aidoo M | 10.1186/s12936-021-03739-6 |
| 34702923 | Epidemiology of mutant Plasmodium falciparum parasites lacking histidine-richprotein 2/3 genes in Eritrea 2 years after switching from HRP2-based RDTs. | Mihreteab S, Anderson K, ..., Cheng Q | 10.1038/s41598-021-00714-8 |
| 35114396 | Pfhrp2/3 gene deletion and genetic variation in PfHRP2-based RDTs with P.falciparum positive samples from India and its implication on malaria control. | Kumari MS, Sharma S, ..., Anvikar AR | 10.1016/j.meegid.2022.105232 |
| 35439987 | Low prevalence of Plasmodium falciparum parasites lacking pfhrp2/3 genes amongasymptomatic and symptomatic school-age children in Kinshasa, Democratic Republicof Congo. | Nundu SS, Arima H, ..., Yamamoto T | 10.1186/s12936-022-04153-2 |
| 37754284 | Increasing Prevalence of Artemisinin-Resistant HRP2-Negative Malaria in Eritrea. | Mihreteab S, Platon L, ..., Menard D | 10.1056/NEJMoa2210956 |
| 37963222 | Force Protection Risks in AFRICOM, INDOPACOM and SOUTHCOM Due to Rapid DiagnosticTest Failures for Falciparum Malaria, 2016-2022 | Vesely BA, Cheng Q | https://pubmed.ncbi.nlm.nih.gov/37963222/ |
| 38098124 | Prediction of malaria positivity using patients' demographic and environmentalfeatures and clinical symptoms to complement parasitological confirmation beforetreatment | Ojurongbe TA, Afolabi HA, ..., Adegoke NA | 10.1186/s40794-023-00208-7 |
| 37886535 | Welcome to the next generation of Malaria Rapid Diagnostic Tests: ComparativeAnalysis of NxTek Eliminate Malaria P.f, Biocredit Malaria Ag Pf, and SD BiolineMalaria Ag Pf for Plasmodium falciparum Diagnosis in Ghana | Kayode TA, Addo AKA, ..., Koepfli C | 10.21203/rs.3.rs-3459263/v1 |
| 38196592 | Expansion of Artemisinin Partial Resistance Mutations and Lack of Histidine RichProtein-2 and -3 Deletions in Plasmodium falciparum infections from Rukara,Rwanda | Schreidah C, Giesbrecht D, ..., Karema C | 10.1101/2023.12.17.23300081 |
| 38157513 | Artemisinin-Resistant HRP2-Negative Malaria in Eritrea. Reply | Menard D, Mihreteab S, Fidock DA | 10.1056/NEJMc2312559#sa2 |
| 38017455 | Assessing the histidine-rich protein 2/3 gene deletion in Plasmodium falciparumisolates from Burkina Faso | Tarama CW, Sore H, ..., Gansane A | 10.1186/s12936-023-04796-9 |
| 38157512 | Artemisinin-Resistant HRP2-Negative Malaria in Eritrea | Bayih AG, Feleke SM, Pillai DR | 10.1056/NEJMc2312559#sa1 |
| 37944271 | Rapid diagnostic tests for malaria diagnosis in Cameroon: impact of histidinerich protein 2/3 deletions and lactate dehydrogenase gene polymorphism | Nana RRD, Ngum NL, ..., Singh V | 10.1016/j.diagmicrobio.2023.116103 |
| 37905102 | Global risk of selection and spread of Plasmodium falciparum histidine-richprotein 2 and 3 gene deletions | Watson OJ, Tran TN, ..., Cunningham J | 10.21.23297352 |
| 37974257 | Estimation of parasitaemia in imported falciparum malaria using the results of acombined rapid diagnostic test. No big help from haematological parameters | Soriano-Perez MJ, Castillo-Fernandez N, ..., Salas-Coronas J | 10.1186/s12936-023-04781-2 |
| 38350136 | Benefits of Lot Testing to Improve the Quality of Malaria Rapid Diagnostic Testsin India | Srivastava B, Sharma S, ..., Anvikar AR | 10.4269/ajtmh.23-0339 |
| 38397717 | Evaluation of the Performance of Rapid Diagnostic Tests for Malaria Diagnosis andMapping of Different Plasmodium Species in Mali | Dembele P, Cissoko M, ..., Ranque S | 10.3390/ijerph21020228 |
| 38037186 | Evaluation of the performance of advantage P.f. malaria Card((R)) and advantagemalaria Pan + Pf Card((R)), two rapid diagnostic tests for parasitologicalconfirmation of malaria cases in field situation in Togo | Teou DC, Dorkenoo AM, ..., Agbonon A | 10.1186/s13071-023-06062-y |
| 37981691 | Diagnostic performance of ultrasensitive rapid diagnostic test for the detectionof Plasmodium falciparum infections in asymptomatic individuals in Kisangani,Northeast Democratic Republic of Congo | Lupaka M, Degefa T, ..., Yewhalaw D | 10.1186/s12936-023-04790-1 |
| 37879855 | Diagnostic value of rapid test for malaria among febrile neonates in a tertiaryhospital in North-East Nigeria: a prospective cross-sectional study | Adeniji YR, Jalo I, ..., Warnow EI | 10.1136/archdischild-2023-325906 |
| 38167003 | Limited threat of Plasmodium falciparum pfhrp2 and pfhrp3 gene deletion to theutility of HRP2-based malaria RDTs in Northern Uganda | Agaba BB, Smith D, ..., Cheng Q | 10.1186/s12936-023-04830-w |
| 38337778 | Assessment of the Performance of Lactate Dehydrogenase-Based Rapid DiagnosticTest for Malaria in Djibouti in 2022-2023 | Abdi Moussa R, Papa Mze N, ..., Bogreau H | 10.3390/diagnostics14030262 |
| 37861484 | Prevalence of Plasmodium species in Badr Governorate, Madinah Province, SaudiArabia using microscopy and rapid diagnostic test | Hassanein RAM, Alkurbi MO, Alsobhi SH | 10.1097/MD.0000000000035516 |