Product Description: serine/threonine protein kinase, FIKK family
SignalP Peptide: MLILMRMLIFASLIIVFINVEN
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0043657 | host cell |
| Component | GO:0005634 | nucleus |
| Function | GO:0005524 | ATP binding |
| Function | GO:0004672 | protein kinase activity |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | possibly expressed | 0.21 | 0.89 |
| Trophozoite | expressed | 0.29 | 0.84 |
| Schizont | possibly expressed | 0.03 | 0.98 |
| Gametocyte | possibly expressed | 0.02 | 0.99 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0902000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.845 | MFS: -3.029 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H513
"Best" AlphaFill ligand hit: B49 (sunitinib, Local RMSD=0.04) with 2Y7J (Global RMSD=5.77)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 11 | 6 | 21 |
| disruptive | 21 | 41 | 22 | 57 |
| missense | 21 | 37 | 21 | 50 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 6 | 24 | 14 | 25 |
| disruptive | 39 | 61 | 46 | 108 |
| missense | 30 | 51 | 40 | 84 |
PlasmoDB Total SNPs: 102
Non-coding: 75 | Synonymous: 7 | Nonsynonymous: 20 | Stop Codon: 0
Protein Length: 542 | Molecular Weight (kDa): 63.978
UniProt ID(s): C0H513
PDB ID(s): None
Isoelectric Point: 9.43
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008266 | Tyrosine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 31650539 | Identification of essential exported Plasmodium falciparum protein kinases inmalaria-infected red blood cells. | Siddiqui G, Proellochs NI, Cooke BM | 10.1111/bjh.16219 |
| 33486853 | Plasmodium falciparum FIKK9.1 is a monomeric serine-threonine protein kinase withfeatures to exploit as a drug target. | D AK, Shrivastava D, ..., Trivedi V | 10.1111/cbdd.13821 |
| 37476548 | Plasmodium falciparum FIKK 9.1 kinase modeling to screen and identify potentantimalarial agents from chemical library | Kumar DA, Karjee P, ..., Trivedi V | 10.1007/s13205-023-03677-x |