PF3D7_0902300 (FIKK9.4)

Genome location: Pf3D7_09_v3:101,962..105,349(-)

Genome classification: Core

Function and Localization

Product Description: serine/threonine protein kinase, FIKK family

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0004674	protein serine/threonine kinase activity
Process	GO:0035556	intracellular signal transduction
Process	GO:0006468	protein phosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	1.27	0.50
Trophozoite	expressed	0.21	0.89
Schizont	expressed	0.19	0.90
Gametocyte	expressed	0.11	0.93

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0902300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.926 | MFS: -2.806 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)

Relative Growth Rate: 0.30 ± 0.11
Confidence: 5.77

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3C5

"Best" AlphaFill ligand hit: ADE (adenine, Local RMSD=0.16) with 7BJE (Global RMSD=9.58)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_111291

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	23	10	28
disruptive	7	60	22	72
missense	7	59	22	56

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	39	14	32
disruptive	27	89	51	113
missense	23	80	45	83

PlasmoDB Total SNPs: 150

Non-coding: 106 | Synonymous: 28 | Nonsynonymous: 16 | Stop Codon: 0

Protein Information

Protein Length: 608 | Molecular Weight (kDa): 72.459

UniProt ID(s): Q8I3C5

PDB ID(s): None

Isoelectric Point: 8.96

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	PF00069	Protein kinase domain
Superfamily	SSF56112	Protein kinase-like domain superfamily