Genome location: Pf3D7_09_v3:108,747..112,128(-)
Genome classification: Core
Product Description: serine/threonine protein kinase, FIKK family
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020036 | Maurer's cleft |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 1.38 | 0.44 |
| Trophozoite | not expressed | 0.21 | 0.89 |
| Schizont | possibly expressed | 0.18 | 0.89 |
| Gametocyte | not expressed | 0.12 | 0.93 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0902500
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.994 | MFS: -1.062 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3C3
"Best" AlphaFill ligand hit: STU (staurosporine, Local RMSD=0.46) with 5E8X (Global RMSD=10.61)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 15 | 5 | 22 |
| disruptive | 49 | 52 | 26 | 53 |
| missense | 31 | 31 | 21 | 32 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 23 | 17 | 31 |
| disruptive | 67 | 94 | 43 | 119 |
| missense | 40 | 57 | 38 | 71 |
PlasmoDB Total SNPs: 136
Non-coding: 67 | Synonymous: 32 | Nonsynonymous: 37 | Stop Codon: 0
Protein Length: 591 | Molecular Weight (kDa): 70.066
UniProt ID(s): Q8I3C3
PDB ID(s): None
Isoelectric Point: 8.36
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008266 | Tyrosine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 17181785 | A novel protein kinase family in Plasmodium falciparum is differentiallytranscribed and secreted to various cellular compartments of the host cell. | Nunes MC, Goldring JP, Doerig C, Scherf A | 10.1111/j.1365-2958.2006.05521.x |