Product Description: ATP-dependent RNA helicase DDX60, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.6.4.13 (RNA helicase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005634 | nucleus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 1.98 | 0.25 |
| Trophozoite | expressed | 1.85 | 0.22 |
| Schizont | expressed | 0.82 | 0.57 |
| Gametocyte | not expressed | 0.83 | 0.59 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0903400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.125 | MFS: -3.11 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0418000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3B4
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.07) with 2XGJ (Global RMSD=3.02)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.6.4.13 | RNA helicase | ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_105684
Most Similar Human Ortholog: D6RBA2
TM-align score: 0.67 | RMSD: 2.63
Seq Identity: 0.20 | Length: 105 / 2536
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000137628 | DExD/H-box helicase 60 |
| ENSG00000181381 | DExD/H-box 60 like |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 39 | 148 | 84 | 156 |
| disruptive | 69 | 313 | 172 | 372 |
| missense | 64 | 308 | 166 | 346 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 60 | 270 | 104 | 176 |
| disruptive | 113 | 611 | 256 | 514 |
| missense | 98 | 567 | 240 | 403 |
PlasmoDB Total SNPs: 433
Non-coding: 240 | Synonymous: 96 | Nonsynonymous: 97 | Stop Codon: 0
Protein Length: 2536 | Molecular Weight (kDa): 300.925
UniProt ID(s): Q8I3B4
PDB ID(s): None
Isoelectric Point: 6.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR011545 | DEAD/DEAH box helicase domain |
| InterPro | IPR014001 | Helicase superfamily 1/2, ATP-binding domain |
| InterPro | IPR027417 | P-loop containing nucleoside triphosphate hydrolase |
| PFam | PF00270 | DEAD/DEAH box helicase domain |
| Superfamily | SSF52540 | P-loop containing nucleoside triphosphate hydrolase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |