Product Description: replication protein A1, small fragment
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005662 | DNA replication factor A complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0003684 | damaged DNA binding |
| Function | GO:0043565 | sequence-specific DNA binding |
| Function | GO:0003697 | single-stranded DNA binding |
| Function | GO:0043047 | single-stranded telomeric DNA binding |
| Process | GO:0006281 | DNA repair |
| Process | GO:0006268 | DNA unwinding involved in DNA replication |
| Process | GO:0006261 | DNA-dependent DNA replication |
| Process | GO:0000724 | double-strand break repair via homologous recombination |
| Process | GO:0051321 | meiotic cell cycle |
| Process | GO:0006289 | nucleotide-excision repair |
| Process | GO:0000723 | telomere maintenance |
| Process | GO:0007004 | telomere maintenance via telomerase |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.11 | 0.95 |
| Trophozoite | expressed | 0.86 | 0.54 |
| Schizont | expressed | 0.33 | 0.79 |
| Gametocyte | expressed | 0.60 | 0.67 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0904800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.124 | MFS: -2.834 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0419400)
Modification: Tagged | RMgm-4982
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I3A1
"Best" AlphaFill ligand hit: SAM (s-adenosylmethionine, Local RMSD=11.97) with 6EFN (Global RMSD=19.41)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P27694 | Replication protein A 70 kDa DNA-binding subunit | OMA | US10774063, Compound NG-01-24 |
Protein Length: 484 | Molecular Weight (kDa): 56.235
UniProt ID(s): Q8I3A1
PDB ID(s): None
Isoelectric Point: 7.7
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR013955 | Replication factor A, C-terminal |
| InterPro | IPR031657 | Replication protein A, OB domain |
| PFam | PF08646 | Replication factor A, C-terminal |
| PFam | PF16900 | Replication protein A, OB domain |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 33 | 19 | 27 |
| disruptive | 3 | 11 | 9 | 32 |
| missense | 3 | 11 | 9 | 24 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 67 | 21 | 29 |
| disruptive | 8 | 48 | 26 | 76 |
| missense | 5 | 33 | 14 | 48 |
PlasmoDB Total SNPs: 124
Non-coding: 92 | Synonymous: 27 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 12368867 | Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. | Hall N, Pain A, ..., Barrell BG | 10.1038/nature01095 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 23631919 | Opposing roles for two molecular forms of replication protein A inRad51-Rad54-mediated DNA recombination in Plasmodium falciparum. | Gopalakrishnan AM, Kumar N | 10.1128/mBio.02515-14 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |
| 37272974 | Differential expression of meiosis and homologous recombination-related genes inthe life cycle of Trypanosoma cruzi | Cruz-Saavedra L, Caceres T, ..., Ramirez JD | 10.1007/s00436-023-07850-2 |
| 37474515 | Identification of a small-molecule inhibitor that selectively blocks DNA-bindingby Trypanosoma brucei replication protein A1 | Mukherjee A, Hossain Z, ..., Kim HS | 10.1038/s41467-023-39839-x |
| 37200372 | Comprehensive mutational analysis of the checkpoint signaling function ofRpa1/Ssb1 in fission yeast | Xu YJ, Bhadra S, ..., Nakamura TM | 10.1371/journal.pgen.1010691 |