About Gene List

PF3D7_0904900 (CuTP)

Genome location: Pf3D7_09_v3:224,609..233,976(-)

Genome classification: Core

Function and Localization

Product Description: copper-transporting ATPase

SignalP Peptide: N/A

# Transmembrane Domains: 7

EC Numbers: 3.6.3.4 (Transferred entry: 7.2.2.9)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0031410 cytoplasmic vesicle
Component GO:0020002 host cell plasma membrane
Component GO:0005886 plasma membrane
Function GO:0016887 ATP hydrolysis activity
Function GO:0005388 P-type calcium transporter activity
Function GO:0008553 P-type proton-exporting transporter activity
Function GO:0005507 copper ion binding
Process GO:0070588 calcium ion transmembrane transport
Process GO:0006874 cellular calcium ion homeostasis
Process GO:0006825 copper ion transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.24 0.89
Trophozoite expressed 1.39 0.40
Schizont expressed 0.89 0.61
Gametocyte possibly expressed 0.65 0.66

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0904900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.503 | #Insertions: 6

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0416500)

Modification: Tagged | RMgm-961

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I3A0

"Best" AlphaFill ligand hit: AMP (adenosine monophosphate, Local RMSD=0.04) with 2YJ4 (Global RMSD=2.75)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.3.4 Cu2+-exporting ATPase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_100289

Most Similar Human Ortholog: A0A2R8YD60

TM-align score: 0.53 | RMSD: 2.95

Seq Identity: 0.11 | Length: 44 / 2568

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000123191 ATPase copper transporting beta
ENSG00000165240 ATPase copper transporting alpha

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 31 139 96 168
disruptive 86 432 260 534
missense 72 384 237 474

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 49 289 120 170
disruptive 168 849 372 629
missense 119 743 322 498

PlasmoDB Total SNPs: 511

Non-coding: 113 | Synonymous: 203 | Nonsynonymous: 195 | Stop Codon: 0

Protein Information

Protein Length: 2568 | Molecular Weight (kDa): 299.39

UniProt ID(s): Q8I3A0

PDB ID(s): None

Isoelectric Point: 5.48

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001757 P-type ATPase
InterPro IPR008250 P-type ATPase, A domain superfamily
InterPro IPR018303 P-type ATPase, phosphorylation site
InterPro IPR023298 P-type ATPase, transmembrane domain superfamily
InterPro IPR036412 HAD-like superfamily
PFam PF00122 N/A
PFam PF00702
Superfamily SSF56784 HAD-like superfamily
Superfamily SSF81653 P-type ATPase, A domain superfamily
Superfamily SSF81665 P-type ATPase, transmembrane domain superfamily

Associated Publications

PMID Title Authors DOI/Link
12368867 Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13. Hall N, Pain A, ..., Barrell BG 10.1038/nature01095
15125686 Copper pathways in Plasmodium falciparum infected erythrocytes indicate an effluxrole for the copper P-ATPase. Rasoloson D, Shi L, ..., Sullivan DJ 10.1042/BJ20040335
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
24237419 Copper-transporting ATPase is important for malaria parasite fertility. Kenthirapalan S, Waters AP, Matuschewski K, Kooij TW 10.1111/mmi.12461