PF3D7_0905100 (NUP221)

Genome location: Pf3D7_09_v3:236,028..243,109(-)

Genome classification: Core

Function and Localization

Product Description: nucleoporin NUP221, putative

SignalP Peptide: N/A

# Transmembrane Domains: 4

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0034399	nuclear periphery
Component	GO:0005643	nuclear pore
Component	GO:0005634	nucleus
Component	GO:0020003	symbiont-containing vacuole

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.84	0.61
Trophozoite	expressed	0.71	0.60
Schizont	expressed	0.45	0.72
Gametocyte	expressed	0.43	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0905100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 3 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.154 | MFS: -2.808 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0416300)

Relative Growth Rate: 0.12 ± 0.18
Confidence: 4.78

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0416300)

Modification: Tagged | RMgm-5242

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I398

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_121194

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	18	114	66	141
disruptive	46	231	115	318
missense	27	205	112	284

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	40	232	92	142
disruptive	91	427	210	461
missense	55	380	180	355

PlasmoDB Total SNPs: 161

Non-coding: 42 | Synonymous: 73 | Nonsynonymous: 46 | Stop Codon: 0

Protein Information

Protein Length: 2112 | Molecular Weight (kDa): 235.538

UniProt ID(s): Q8I398

PDB ID(s): None

Isoelectric Point: 8.45

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR025574	Nucleoporin FG repeat
PFam	PF13634	Nucleoporin FG repeat
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
12368867	Sequence of Plasmodium falciparum chromosomes 1, 3-9 and 13.	Hall N, Pain A, ..., Barrell BG	10.1038/nature01095
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
19765590	Potential epigenetic regulatory proteins localise to distinct nuclearsub-compartments in Plasmodium falciparum.	Volz J, Carvalho TG, ..., Cowman AF	10.1016/j.ijpara.2009.09.002
20419108	A moonlighting function of Plasmodium falciparum histone 3, mono-methylated atlysine 9?	Luah YH, Chaal BK, Ong EZ, Bozdech Z	10.1371/journal.pone.0010252
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
38405843	Nuclear pore complexes undergo Nup221 exchange during blood stage asexualreplication of Plasmodium parasites	Blauwkamp JA, Ambekar SV, ..., Absalon S	10.1101/2024.02.16.580747